RESUMEN
Schistosomiasis is one of the most important tropical diseases. A fundamental strategy to control its spread is the use of natural products against its vectors, which are snails of the genus Biomphalaria. The present study evaluated the chemical composition, the molluscicidal and cercaricidal effects, and the ecotoxicity of the essential oil from the aerial parts of Dysphania ambrosioides (L.) Mosyakin & Clemants (DAEO). The essential oil was obtained by hydrodistillation and analyzed by gas chromatography-mass spectrometry (GC-MS). Molluscicidal and cercaricidal activities were determined by the immersion method. Environmental toxicity was assessed from bioassays using Artemia salina larvae and Danio rerio fish. DAEO presented a 0.8% yield. The GC-MS analysis revealed the predominance of hydrocarbon monoterpenes in the oil. A total of 32 constituents was identified, with α-terpinene (50.69%) being the major compound, followed by p-cymene (13.27%) and ascaridole (10.26%). DAEO was active against adult Biomphalaria glabrata snails and demonstrated lethal effect against Schistosoma mansoni cercariae, with LC50 values of 25.2 (22.7-27.8) and 62.4 (61.8-62.9) µg/mL, respectively. Regarding toxicity to non-target aquatic organisms, the oil showed LC50 values of 86.9 (84.7-87.6) and 18.6 µg/mL (15.5-22.8) for A. salina and D. rerio, respectively. DAEO proved to be a promising natural product for the control of schistosomiasis, acting on both the vectors and the etiological agent of the disease. However, the use of the oil is safer in transmission sites where there are no non-target organisms, as it has showed toxicity to D. rerio fish.
Asunto(s)
Biomphalaria , Moluscocidas , Aceites Volátiles , Esquistosomiasis , Animales , Moluscocidas/química , Moluscocidas/toxicidad , Aceites Volátiles/toxicidad , Schistosoma mansoni , Pez CebraRESUMEN
This research reports, for the first time, the immobilization of an enzyme - Rhus vernificera laccase - on cashew gum (CG) nanoparticles (NPs) and its application as a biological layer in the design and development of an electrochemical biosensor. Laccase-CG nanoparticles (LacCG-NPs) were prepared by the nanoprecipitation method and characterized by UV-Vis spectrophotometry, atomic force microscopy, scanning electron microscopy, attenuated total reflectance-Fourier-transform infrared spectroscopy, circular dichroism, cyclic voltammetry, and electrochemical impedance spectroscopy. The average size and stability of the NPs were predicted by DLS and zeta potential. The ATR-FTIR results clearly demonstrated an interaction between -NH and -OH groups to form LacCG-NPs. The average size found for LacCG-NPs was 280 ± 53 nm and a polydispersity index of 0.309 ± 0.08 indicated a good particle size distribution. The zeta potential shows a good colloidal stability. The use of a natural product to prepare the enzymatic nanoparticles, its easy synthesis and the immobilization efficiency should be highlighted. LacCG-NPs were successfully applied as a biolayer in the development of an amperometric biosensor for catechol detection. The resulting device showed a low response time (6 s), good sensitivity (7.86 µA µM-1 cm-2), wide linear range of 2.5 × 10-7-2.0 × 10-4 M, and low detection limit (50 nM).
Asunto(s)
Materiales Biocompatibles/química , Técnicas Biosensibles , Catecoles/análisis , Lacasa/química , Nanopartículas/química , Gomas de Plantas/química , Anacardium/química , Materiales Biocompatibles/síntesis química , Materiales Biocompatibles/metabolismo , Conformación de Carbohidratos , Técnicas Electroquímicas , Lacasa/metabolismo , Ensayo de Materiales , Modelos Moleculares , Nanopartículas/metabolismo , Tamaño de la Partícula , Gomas de Plantas/aislamiento & purificación , Gomas de Plantas/metabolismo , Toxicodendron/enzimologíaRESUMEN
In this study, nanoemulsions of essential oil from Ocimumgratissimum (Linn) (EO) were produced using low and high energy techniques using cashew gum (CG) as a co-surfactant. The main constituents of the EO were determined by Gas Chromatography coupled with Mass Spectrometry (GC-MS), and their presence in the EO and in the formulations verified by Fourier Transform Infrared Spectroscopy (FTIR) and UV-visible spectrophotometry was observed the encapsulation efficiency (EE%), with colloidal stability. Nuclear magnetic resonance (NMR) was used to study cashew gum. Dynamic light scattering analysis (DLS) determined the nanoemulsion Z means, polydispersity index and the Zeta potential value, nanoparticle tracking analysis (NTA) were determined. The nanostructured EO showed better antibacterial action against the pathogenic gastroenteritis species Staphylococcus aureus, Escherichia coli and Salmonella enterica when compared to free EO. Atomic Force Microscopy (AFM) was used for morphological analysis of the nanoparticle and study of the action of the nanoemulsion through images of the cellular morphology of S. enterica. The antioxidant activity was evaluated against the ABTS radical (2,2'-azino-bis diazonium salt (3-ethylbenzothiazoline-6-sulfonic acid)). The encapsulation of EO in a nanostructured system improved its antibacterial and antioxidant activity, the low energy synthesis showed greater storage stability, remaining stable for 37 days.
Asunto(s)
Antibacterianos/química , Emulsiones/química , Ocimum/metabolismo , Aceites Volátiles/química , Gomas de Plantas/química , Hojas de la Planta/metabolismoRESUMEN
The use of bisphosphonates constitutes the gold-standard therapy for the control and treatment of bone diseases. However, its long-term use may lead to gastric problems, which limits the treatment. Thus, this study aimed to formulate a nanostructured system with biodegradable polymers for the controlled release of alendronate sodium. The nanoparticles were characterized, and its gastric toxicity was investigated in rats. The synthesis process proved to be effective for encapsulating alendronate sodium, exhibiting nanoparticles with an average size of 51.02 nm and 98.5% of alendronate sodium incorporation. The release tests demonstrated a controlled release of the drug in 420 min, while the morphological analyzes showed spherical shapes and no apparent roughness. The biological tests demonstrated that the alendronate sodium nanoformulation reversed the gastric lesions, maintaining the normal levels of malondialdehyde and myeloperoxidase. Also, the encapsulated alendronate sodium showed no toxicity in murine osteoblastic cells, even at high concentrations.
Asunto(s)
Alendronato , Nanopartículas , Alendronato/toxicidad , Animales , Preparaciones de Acción Retardada/farmacología , Mucosa Gástrica , Ratones , Nanopartículas/toxicidad , Polímeros/farmacología , RatasRESUMEN
Latex, a milky fluid found in several plants, is widely used for many purposes, and its proteins have been investigated by researchers. Many studies have shown that latex produced by some plant species is a natural source of biologically active compounds, and many of the hydrolytic enzymes are related to health benefits. Research on the characterization and industrial and pharmaceutical utility of latex has progressed in recent years. Latex proteins are associated with plants' defense mechanisms, against attacks by fungi. In this respect, there are several biotechnological applications of antifungal proteins. Some findings reveal that antifungal proteins inhibit fungi by interrupting the synthesis of fungal cell walls or rupturing the membrane. Moreover, both phytopathogenic and clinical fungal strains are susceptible to latex proteins. The present review describes some important features of proteins isolated from plant latex which presented in vitro antifungal activities: protein classification, function, molecular weight, isoelectric point, as well as the fungal species that are inhibited by them. We also discuss their mechanisms of action.
Asunto(s)
Antifúngicos/farmacología , Quitinasas/farmacología , Látex/química , Péptido Hidrolasas/farmacología , Peroxidasas/farmacología , Lectinas de Plantas/farmacología , Proteínas de Plantas/farmacología , Antifúngicos/clasificación , Antifúngicos/aislamiento & purificación , Botrytis/efectos de los fármacos , Botrytis/crecimiento & desarrollo , Candida albicans/efectos de los fármacos , Candida albicans/crecimiento & desarrollo , Quitinasas/clasificación , Quitinasas/aislamiento & purificación , Quitinasas/fisiología , Fusarium/efectos de los fármacos , Fusarium/crecimiento & desarrollo , Punto Isoeléctrico , Pruebas de Sensibilidad Microbiana , Peso Molecular , Péptido Hidrolasas/clasificación , Péptido Hidrolasas/aislamiento & purificación , Péptido Hidrolasas/fisiología , Peroxidasas/clasificación , Peroxidasas/aislamiento & purificación , Peroxidasas/fisiología , Enfermedades de las Plantas/microbiología , Extractos Vegetales/química , Lectinas de Plantas/clasificación , Lectinas de Plantas/aislamiento & purificación , Lectinas de Plantas/fisiología , Proteínas de Plantas/clasificación , Proteínas de Plantas/aislamiento & purificación , Proteínas de Plantas/fisiología , Plantas/químicaRESUMEN
This study presents a green synthesis route to silver nanoparticles (AgNPs) stabilized with cashew gum (CG) or carboxymethylated cashew gum (CCG) using microwave-assisted synthesis and evaluates their antibacterial activity. The antimicrobial activity was measured by determining the minimum inhibitory concentration (MIC) with Staphylococcus aureus and Escherichia coli. In both cases of the presence of CG and CCG, it was found that higher pH lead to more efficient conversion of silver nitrate to AgNPs with well dispersed, spherical and stable particles as well as low crystallinity. CCG-capped AgNPs were slightly smaller (137.0 and 96.3 nm) than those coated with non-modified gum (144.7 and 100.9 nm). The samples presented promising antibacterial activity, especially on Gram-negative bacteria, resulting in significant membrane damage on treated bacteria in comparison to the untreated control, observed by atomic force microscopy. Thus, a quick and efficient synthesis route was applied to produce CGAgNPs and CCGAgNPs with antimicrobial potential.