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1.
J Immunol ; 186(4): 2503-13, 2011 Feb 15.
Artículo en Inglés | MEDLINE | ID: mdl-21239719

RESUMEN

Bortezomib, an inhibitor of proteasomes, has been reported to reduce autoantibody titers and to improve clinical condition in mice suffering from lupus-like disease. Bortezomib depletes both short- and long-lived plasma cells; the latter normally survive the standard immunosuppressant treatments targeting T and B cells. These findings encouraged us to test whether bortezomib is effective for alleviating the symptoms in the experimental autoimmune myasthenia gravis (EAMG) model for myasthenia gravis, a disease that is characterized by autoantibodies against the acetylcholine receptor (AChR) of skeletal muscle. Lewis rats were immunized with saline (control, n = 36) or Torpedo AChR (EAMG, n = 54) in CFA in the first week of an experimental period of 8 wk. After immunization, rats received twice a week s.c. injections of bortezomib (0.2 mg/kg in saline) or saline injections. Bortezomib induced apoptosis in bone marrow cells and reduced the amount of plasma cells in the bone marrow by up to 81%. In the EAMG animals, bortezomib efficiently reduced the rise of anti-AChR autoantibody titers, prevented ultrastructural damage of the postsynaptic membrane, improved neuromuscular transmission, and decreased myasthenic symptoms. This study thus underscores the potential of the therapeutic use of proteasome inhibitors to target plasma cells in Ab-mediated autoimmune diseases.


Asunto(s)
Autoanticuerpos/efectos de los fármacos , Ácidos Borónicos/farmacología , Miastenia Gravis Autoinmune Experimental/tratamiento farmacológico , Miastenia Gravis Autoinmune Experimental/inmunología , Células Plasmáticas/efectos de los fármacos , Inhibidores de Proteasas/farmacología , Inhibidores de Proteasoma , Pirazinas/farmacología , Animales , Autoanticuerpos/biosíntesis , Bortezomib , Femenino , Depleción Linfocítica/métodos , Miastenia Gravis Autoinmune Experimental/enzimología , Células Plasmáticas/enzimología , Células Plasmáticas/patología , Ratas , Ratas Endogámicas Lew
2.
Sci Rep ; 7(1): 992, 2017 04 20.
Artículo en Inglés | MEDLINE | ID: mdl-28428630

RESUMEN

Autoantibodies against ion channels are the cause of numerous neurologic autoimmune disorders. Frequently, such pathogenic autoantibodies have a restricted epitope-specificity. In such cases, competing antibody formats devoid of pathogenic effector functions (blocker antibodies) have the potential to treat disease by displacing autoantibodies from their target. Here, we have used a model of the neuromuscular autoimmune disease myasthenia gravis in rhesus monkeys (Macaca mulatta) to test the therapeutic potential of a new blocker antibody: MG was induced by passive transfer of pathogenic acetylcholine receptor-specific monoclonal antibody IgG1-637. The effect of the blocker antibody (IgG4Δhinge-637, the hinge-deleted IgG4 version of IgG1-637) was assessed using decrement measurements and single-fiber electromyography. Three daily doses of 1.7 mg/kg IgG1-637 (cumulative dose 5 mg/kg) induced impairment of neuromuscular transmission, as demonstrated by significantly increased jitter, synaptic transmission failures (blockings) and a decrease in the amplitude of the compound muscle action potentials during repeated stimulations (decrement), without showing overt symptoms of muscle weakness. Treatment with three daily doses of 10 mg/kg IgG4Δhinge-637 significantly reduced the IgG1-637-induced increase in jitter, blockings and decrement. Together, these results represent proof-of principle data for therapy of acetylcholine receptor-myasthenia gravis with a monovalent antibody format that blocks binding of pathogenic autoantibodies.


Asunto(s)
Autoanticuerpos/metabolismo , Inmunoglobulina G/administración & dosificación , Miastenia Gravis/tratamiento farmacológico , Receptores Colinérgicos/metabolismo , Animales , Células CHO , Antagonistas Colinérgicos , Cricetulus , Modelos Animales de Enfermedad , Regulación de la Expresión Génica/efectos de los fármacos , Células HEK293 , Exones de la Región Bisagra , Humanos , Inmunoglobulina G/farmacología , Macaca mulatta , Miastenia Gravis/inmunología , Miastenia Gravis/metabolismo , Transmisión Sináptica/efectos de los fármacos , Resultado del Tratamiento
3.
Autoimmunity ; 43(5-6): 353-70, 2010 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-20380584

RESUMEN

Myasthenia gravis (MG) is an autoimmune disorder caused by autoantibodies that are either directed to the muscle nicotinic acetylcholine receptor (AChR) or to the muscle-specific tyrosine kinase (MuSK). These autoantibodies define two distinct subforms of the disease-AChR-MG and MuSK-MG. Both AChR and MuSK are expressed on the postsynaptic membrane of the neuromuscular junction (NMJ), which is a highly specialized region of the muscle dedicated to receive and process signals from the motor nerve. Autoantibody binding to proteins of the postsynaptic membrane leads to impaired neuromuscular transmission and muscle weakness. Pro-inflammatory antibodies of the human IgG1 and IgG3 subclass modulate the AChR, cause complement activation, and attract lymphocytes; together acting to decrease levels of the AChR and AChR-associated proteins and to reduce postsynaptic folding. In patients with anti-MuSK antibodies, there is no evidence of loss of junctional folds and no apparent loss of AChR density. Anti-MuSK antibodies are predominantly of the IgG4 isotype, which functionally differs from other IgG subclasses in its anti-inflammatory activity. Moreover, IgG4 undergoes a posttranslational modification termed Fab arm exchange that prevents cross-linking of antigens. These findings suggest that MuSK-MG may be different in etiological and pathological mechanisms from AChR-MG. The effector functions of IgG subclasses on synapse structure and function are discussed in this review.


Asunto(s)
Autoanticuerpos/inmunología , Miastenia Gravis/inmunología , Unión Neuromuscular/inmunología , Unión Neuromuscular/fisiopatología , Proteínas Tirosina Quinasas Receptoras/inmunología , Receptores Colinérgicos/inmunología , Receptores Nicotínicos/inmunología , Animales , Autoanticuerpos/sangre , Humanos , Inmunoglobulina G/sangre , Inmunoglobulina G/inmunología , Debilidad Muscular/fisiopatología , Atrofia Muscular/fisiopatología , Miastenia Gravis/patología , Miastenia Gravis/fisiopatología , Unión Neuromuscular/patología , Proteínas Tirosina Quinasas Receptoras/metabolismo , Receptores Colinérgicos/metabolismo , Receptores Nicotínicos/metabolismo
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