Local Structure and Crystallization Transformation of Hydrous Ferric Arsenate in Acidic H2O-Fe(III)-As(V)-SO42- Systems: Implications for Acid Mine Drainage and Arsenic Geochemical Cycling.

Hydrous ferric arsenate (HFA) is a common thermodynamically metastable phase in acid mine drainage (AMD). However, little is known regarding the structural forms and transformation mechanism of HFA. We investigated the local atomic structures and the crystallization transformation of HFA at various Fe(III)/As(V) ratios (2, 1, 0.5, 0.33, and 0.25) in acidic solutions (pH 1.2 and 1.8). The results show that the Fe(III)/As(V) in HFA decreases with decreasing initial Fe(III)/As(V) at acidic pHs. The degree of protonation of As(V) in HFA increases with increasing As(V) concentrations. The Fe K-edge extended X-ray absorption fine structure and X-ray absorption near-edge structure results reveal that each FeO6 is linked to more than two AsO4 in HFA precipitated at Fe(III)/As(V) < 1. Furthermore, the formation of scorodite (FeAsO4·2H2O) is greatly accelerated by decreasing the initial Fe(III)/As(V). The release of As(V) from HFA is observed during its crystallization transformation process to scorodite at Fe(III)/As(V) < 1, which is different from that at Fe(III)/As(V) ≥ 1. Scanning electron microscopy results show that Oswald ripening is responsible for the coarsening of scorodite regardless of the initial Fe(III)/As(V) or pH. Moreover, the formation of crystalline ferric dihydrogen arsenate as an intermediate phase at Fe(III)/As(V) < 1 is responsible for the enhanced transformation rate from HFA to scorodite. This work provides new insights into the local atomic structure of HFA and its crystallization transformation that may occur in AMD and has important implications for arsenic geochemical cycling.

Unraveling the roles of microporous and micro-mesoporous structures of carbon supports on iron oxide properties and As (V) removal performance in contaminated water.

This study investigates the impact of microporous (SP-C) and micro-mesoporous carbon (DP-C) supports on the dispersion and phase transformation of iron oxides and their arsenic (V) removal efficiency. The research demonstrates that carbon-supported iron oxide sorbents exhibit superior As(V) uptake capacity compared to unsupported Fe2O3, attributed to reduced iron oxide crystallite sizes and As(V) adsorption on carbon supports. Maximum As(V) uptake capacities of 23.8 mg/g and 18.9 mg/g were achieved for Fe/SP-C and Fe/DP-C at 30 wt% and 50 wt% iron loading, respectively. The study reveals a nonlinear relationship between As(V) sorption capacity and iron oxide crystallite size after excluding As(V) adsorption capacity on carbon supports, suggesting the iron oxide phase (Fe3O4) plays a role in determining adsorption capacity. Iron oxide-loaded DP-C sorbents exhibit faster adsorption rates at low As(V) concentrations (5 mg/L) than SP-C sorbents due to their bimodal pore structure. Adsorption behavior varies at higher As(V) concentrations (45 mg/L), with Fe/DP-C reaching maximum capacity more slowly due to limited available adsorptive sites. All adsorbents maintained near-complete As(V) removal efficiency over five cycles. The findings provide insights for designing more efficient adsorbents for As(V) removal from contaminated water sources.

Occurrence, speciation, and controls on arsenic mobilization in the alluvial aquifer system of the Ghaghara basin, India.

High concentrations of arsenic (As) in groundwater are among the long-standing environmental problems on the planet. Due to adverse impacts on the human and aquatic system, characterization and quantification of individual inorganic As species are crucial in understanding the occurrence, environmental fate, behaviour, and toxicity in natural waters. This study presents As concentration and its speciation As(III) and As(V) data, including the interrelationship with other major and trace aqueous solutes from parts of the Ghaghara basin, India. More than half (57%) of the groundwater samples exhibited elevated As concentrations (> 10 µg/L), whereas 67.4% of samples have higher As(III) values relative to As(V), signifying a potential risk of As(III) toxicity. The elevated concentration of As was associated with higher Fe, Mn, and HCO3-, especially in samples from shallow well depth. PHREEQC modeling demonstrates the presence of mineral phases such as hematite, goethite, rhodochrosite, etc. Therefore, it is inferred that the release of As from sediment particles into pore water via microbially mediated Fe/Mn oxyhydroxides, and As(V) reduction processes mainly control high As concentrations. The heavy metal pollution indices (HPI) and (HEI) values revealed heavy metal pollution in low-lying areas deposited by relatively younger sediments along the Ghaghara River. Large-scale agricultural practices, overexploitation of groundwater, and indiscriminate sewage disposal, in addition to geogenic factors, cannot be ruled out as potential contributors to As mobilization in the region. This study recommends conducting seasonal hydrogeochemical monitoring and investigating regional natural background levels of As, to precisely understand the controlling mechanistic pathways of As release.

Arsenic release from microbial reduction of scorodite in the presence of electron shuttle in flooded soil.

Scorodite (FeAsO4·H2O) is a common arsenic-bearing (As-bearing) iron mineral in near-surface environments that could immobilize or store As in a bound state. In flooded soils, microbe induced Fe(III) or As(V) reduction can increase the mobility and bioavailability of As. Additionally, humic substances can act as electron shuttles to promote this process. The dynamics of As release and diversity of putative As(V)-reducing bacteria during scorodite reduction have yet to be investigated in detail in flooded soils. Here, the microbial reductive dissolution of scorodite was conducted in an flooded soil in the presence of anthraquinone-2,6-disulfonate (AQDS). Anaeromyxobacter, Dechloromonas, Geothrix, Geobacter, Ideonella, and Zoogloea were found to be the dominant indigenous bacteria during Fe(III) and As(V) reduction. AQDS increased the relative abundance of dominant species, but did not change the diversity and microbial community of the systems with scorodite. Among these bacteria, Geobacter exhibited the greatest increase and was the dominant Fe(III)- and As(V)-reducing bacteria during the incubation with AQDS and scorodite. AQDS promoted both Fe(III) and As(V) reduction, and over 80% of released As(V) was microbially transformed to As(III). The increases in the abundance of arrA gene and putative arrA sequences of Geobacter were higher with AQDS than without AQDS. As a result, the addition of AQDS promoted microbial Fe(III) and As(V) release and reduction from As-bearing iron minerals into the environment. These results contribute to exploration of the transformation of As from As-bearing iron minerals under anaerobic conditions, thus providing insights into the bioremediation of As-contaminated soil.

Adsorption of As(III) and As(V) from aqueous solution by magnetic biosorbents derived from chemical carbonization of pea peel waste biomass: Isotherm, kinetic, thermodynamic and breakthrough curve modeling studies.

The purpose of this research was to investigate the adsorption of arsenic (As) from aqueous solutions using MPAC-500 and MPAC-600 (magnetic-activated carbons synthesized from the peel of Pisum sativum (pea) pyrolyzed at 500 °C and 600 °C temperatures, respectively). The potential of both biosorbents for As adsorption was determined in batch and column mode. The characterization of both biosorbents was performed by energy dispersive spectroscopy, scanning electron microscope, pHZPC, particle size distribution, X-ray diffraction, zeta potential and Fourier-transform infrared spectroscopy. It was found that the efficiency of MPAC-600 was better than MPAC-500 for the adsorption of As(III) and As(V) ions. The adsorption capacities of MPAC-500 and MPAC-600 in removing As(III) were 0.7297 mg/g and 1.3335 mg/g, respectively, while the values of Qmax for As(V) on MPAC-500 and MPAC-600 were 0.4930 mg/g and 0.9451 mg/g, respectively. The Langmuir isotherm model was found to be the best fit for adsorption of As(III) by MPAC-500 and MPAC-600, as well as adsorption of As(V) by MPAC-500. The Freundlich isotherm model, on the other hand, was optimal for As(V) removal with MPAC-600. With R2 values close to unity, the pseudo-second-order kinetics were best fitted to the adsorption process of both As species. The Thomas model was used to estimate the breakthrough curves. The effects of coexisting oxyanions and regeneration studies were also carried out to examine the influence of oxyanions on As adsorption and reusability of biosorbents.

Fabrication of Novel Bentonite-Anthracite@Zetag (BT-An@Zetag) Composite for the Removal of Arsenic (V) from an Aqueous Solution.

The arsenic (As) pollution of water has been eliminated via intensive scientific efforts, with the purpose of giving safe drinking water to millions of people across the world. In this study, the adsorption of As(V) from a synthetic aqueous solution was verified using a Bentonite-Anthracite@Zetag (BT-An@Zetag) composite. The SEM, FT-IR, XRD, DSC, TGA, and SBET techniques were used to characterize the (BT-An@Zetag) composite. The adsorption of As(V) was explored using batch adsorption under varied operating scenarios. Five kinetic modelswere used to investigate kinetic data, whereas three isotherms had been used to fit empirical equilibrium data. According to the findings, the adsorption mechanism of As(V) was best described by the Freundlich isotherm with a maximum monolayer coverage of 38.6 mg/g showing pseudo-second-order mode. The estimated enthalpy (H°) indicates that the adsorption process is both chemical and endothermic.The calculated free energy (G°) indicates that the reaction is nonspontaneous. After four sequential adsorption cycles, the produced BT-An@Zetag composite demonstrated good reusability and a greater adsorption affinity for As(V) ions. Overall, the BT-An@Zetag composite is suited for removing arsenic from wastewater using adsorption as a cost-effective and efficient technique.

Efficiency of arsenic remediation from growth medium through Bacillus licheniformis modulated by phosphate (PO4)3- and nitrate (NO3)- enrichment.

Bacillus licheniformis DAS-1 and DAS-2 were found as potent tool for removal/uptake of arsenic [As(V) and As(III)] and reduction [(As(V) to As(III)] of arsenic from the liquid growth medium in our previous studies. Present work gives light on modulation of arsenic remediation (in terms of uptake and reduction) by two selected essential soil nutrients, phosphate (PO4)3- and nitrate (NO3)-. PO43- has structural analogy with arsenate [AsO43-/As(V)] that compete with cell uptake of As(V). It was found that enrichment of 0.75 mM of PO43- had significantly moderated the As(V) toxicity in liquid broth culture by retarding As(V) uptake. Lowering level of PO43- can lead to increase in As(V) removal from medium and vice versa. NO3- has strong oxidant properties which controls As(III) oxidation into As(V) in medium that resulted less toxicity favouring growth of bacteria and also more uptake via phosphate transporters. Hence, accelerated As(III) uptake has shown on enrichment of 0.5 mM of NO3- in medium. All the results of work give evidence that appropriate enrichment of PO43- and NO3- into liquid growth medium, can significantly contribute in alteration of efficiency for arsenic removal/uptake and reduction by bacteria from the medium.

Synthetic Iowaite Can Effectively Remove Inorganic Arsenic from Marine Extract.

For the removal of arsenic from marine products, iowaite was prepared and investigated to determine the optimal adsorption process of arsenic. Different chemical forms of arsenic (As(III), As(V)) with varying concentrations (0.15, 1.5, 5, 10, 15, and 20 mg/L) under various conditions including pH (3, 5, 7, 9, 11) and contact time (1, 2, 5, 10, 15, 30, 60, 120, 180 min) were exposed to iowaite. Adsorption isotherms and metal ions kinetic modeling onto the adsorbent were determined based on Langmuir, Freundlich, first- and second-order kinetic models. The adsorption onto iowaite varied depending on the conditions. The adsorption rates of standard solution, As(III) and As(V) exceeded 95% under proper conditions, while high complexity was noted with marine samples. As(III) and As(V) from Mactra veneriformis extraction all decreased when exposed to iowaite. The inclusion morphology and interconversion of organic arsenic limit adsorption. Iowaite can be efficiently used for inorganic arsenic removal from wastewater and different marine food products, which maybe other adsorbent or further performance of iowaite needs to be investigated for organic arsenic.

GTPase ROP6 negatively modulates phosphate deficiency through inhibition of PHT1;1 and PHT1;4 in Arabidopsis thaliana.

Phosphorus, an essential macroelement for plant growth and development, is a major limiting factor for sustainable crop yield. The Rho of plant (ROP) GTPase is involved in regulating multiple signal transduction processes in plants, but potentially including the phosphate deficiency signaling pathway remains unknown. Here, we identified that the rop6 mutant exhibited a dramatic tolerant phenotype under Pi-deficient conditions, with higher phosphate accumulation and lower anthocyanin content. In contrast, the rop6 mutant was more sensitive to arsenate (As(V)) toxicity, the analog of Pi. Immunoblot analysis displayed that the ROP6 protein was rapidly degraded through ubiquitin/26S proteasome pathway under Pi-deficient conditions. In addition, pull-down assay using GST-RIC1 demonstrated that the ROP6 activity was decreased obviously under Pi-deficient conditions. Strikingly, protein-protein interaction and two-voltage clamping assays demonstrated that ROP6 physically interacted with and inhibited the key phosphate uptake transporters PHT1;1 and PHT1;4 in vitro and in vivo. Moreover, genetic analysis showed that ROP6 functioned upstream of PHT1;1 and PHT1;4. Thus, we conclude that GTPase ROP6 modulates the uptake of phosphate by inhibiting the activities of PHT1;1 and PHT1;4 in Arabidopsis.

Groundwater As Removal by As(III), Fe(II), and Mn(II) Co-Oxidation: Contrasting As Removal Pathways with O2, NaOCl, and KMnO4.

Effective arsenic (As) removal from groundwater is a pressing need in view of increasingly stringent As drinking water limits in some US states and European countries. In this study, we compared the addition of weak (O2), intermediate (NaOCl), and strong (KMnO4) groundwater oxidants on the fate of As during As(III), Fe(II), and Mn(II) co-oxidation. Experiments were performed with 50 µg/L As(III), 5 mg/L Fe(II), and 0.5 mg/L Mn(II) in solutions containing relevant groundwater ions, with the reaction products characterized by As K-edge X-ray absorption spectroscopy (XAS). Adding O2 by aeration was the least effective method, unable to decrease As to below 10 µg/L, which was attributed to inefficient As(III) oxidation. Dosing NaOCl (55 µM) consistently removed As to <10 µg/L (and often <5 µg/L). The As K-edge XAS data of the NaOCl samples indicated complete As(III) oxidation and As(V) sorption to coprecipitated hydrous ferric oxide (HFO) in the binuclear, bridging (2C) complex. The most effective As removal was observed with KMnO4 (40 µM), which completely oxidized As(III) and yielded residual As concentrations that were less than (by as much as 50%) or equal to the NaOCl experiments. Furthermore, the average As-metal bond length of the KMnO4 solids (RAs-Fe/Mn = 3.24 ± 0.02 Å) was systematically shorter than the NaOCl solids (RAs-Fe/Mn = 3.29 ± 0.02 Å), consistent with As(V) sorption to both MnO2 and HFO. These findings can be used to optimize groundwater As treatment to meet relevant drinking water guidelines, while considering the As uptake mode and characteristics of the particle suspension (i.e., colloidal stability and filterability).

Microbial reactions and environmental factors affecting the dissolution and release of arsenic in the severely contaminated soils under anaerobic or aerobic conditions.

The soils near the abandoned Shimen Realgar Mine are characterized by containing extremely high contents of total and soluble arsenic. To determine the microbial reactions and environmental factors affecting the mobilization and release of arsenic from soils phase into pore water, we collected 24 soil samples from the representative points around the abandoned Shimen Realgar Mine. They contained 8310.84 mg/kg total arsenic and 703.21 mg/kg soluble arsenic in average. The soluble arsenic in the soils shows significant positive and negative correlations with environmental SO42-/TOC/pH/PO43-, and Fe/Mn, respectively. We found that diverse dissimilatory As(V)-respiring prokaryotes (DARPs) and As(III)-oxidizing bacteria (AOB) exist in all the examined soil samples. The activities of DARPs led to 65-1275% increase of soluble As(III) in the examined soils after 21.0 days of anaerobic incubation, and the microbial dissolution and releases of arsenic show significant positive and negative correlations with the environmental pH/TN and NH4+/PO43-, respectively. In comparison, the activities of AOB led to 24-346% inhibition of the dissolved oxygen-mediated dissolution of arsenic in the soils, and the AOB-mediated releases of As(V) show significant positive and negative correlations with the environmental SO42- and pH/NH4+, respectively. The microbial communities of 24 samples contain 54 phyla of bacteria that show extremely high diversities. Total arsenic, TOC, NO3- and pH are the key environmental factors that indirectly controlled the mobilization and release of arsenic via influencing the structures of the microbial communities in the soils. This work gained new insights into the mechanism for how microbial communities catalyze the dissolution and releases of arsenic from the soils with extremely high contents of arsenic.

Synthesis of a novel ternary HA/Fe-Mn oxides-loaded biochar composite and its application in cadmium(II) and arsenic(V) adsorption.

Cadmium (Cd) and arsenic (As) are two of the most toxic elements. However, the chemical behaviors of these two elements are different, making it challenging to utilize a single adsorbent with high adsorption capacity for both Cd(II) and As(V) removal. To solve this problem, we synthesized HA/Fe-Mn oxides-loaded biochar (HFMB), a novel ternary material, to perform this task, wherein scanning electron microscopy (SEM) combined with EDS (SEM-EDS) was used to characterize its morphological and physicochemical properties. The maximum adsorption capacity of HFMB was 67.11 mg/g for Cd(II) and 35.59 mg/g for As(V), which is much higher compared to pristine biochar (11.06 mg/g, 0 mg/g for Cd(II) and As(V), respectively). The adsorption characteristics were investigated by adsorption kinetics and the effects of the ionic strength and pH of solutions. X-ray photoelectron spectroscopy (XPS) and Fourier-transform infrared spectroscopy (FT-IR) revealed that chelation and deposition were the adsorption mechanisms that bound Cd(II) to HFMB, while ligand exchange was the adsorption mechanism that bound As(V).

Changes in biooxidation mechanism and transient biofilm characteristics by As(V) during arsenopyrite colonization with Acidithiobacillus thiooxidans.

Chemical and surface analyses are carried out using Raman spectroscopy, X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM-EDS), atomic force microscopy (AFM), confocal laser scanning microscopy (CLSM), glow discharge spectroscopy (GDS) and extracellular surface protein quantification to thoroughly investigate the effect of supplementary As(V) during biooxidation of arsenopyrite by Acidithiobacillus thiooxidans. It is revealed that arsenic can enhance bacterial reactions during bioleaching, which can strongly influence its mobility. Biofilms occur as compact-flattened microcolonies, being progressively covered by a significant amount of secondary compounds (S n2- , S0, pyrite-like). Biooxidation mechanism is modified in the presence of supplementary As(V), as indicated by spectroscopic and microscopic studies. GDS confirms significant variations between abiotic control and biooxidized arsenopyrite in terms of surface reactivity and amount of secondary compounds with and without As(V) (i.e. 6 µm depth). CLSM and protein analyses indicate a rapid modification in biofilm from hydrophilic to hydrophobic character (i.e. 1-12 h), in spite of the decrease in extracellular surface proteins in the presence of supplementary As(V) (i.e. stressed biofilms).

Facilitative capture of As(V), Pb(II) and methylene blue from aqueous solutions with MgO hybrid sponge-like carbonaceous composite derived from sugarcane leafy trash.

Enhancing the contaminant adsorption capacity is a key factor affecting utilization of carbon-based adsorbents in wastewater treatment and encouraging development of biomass thermo-disposal. In this study, a novel MgO hybrid sponge-like carbonaceous composite (HSC) derived from sugarcane leafy trash was prepared through an integrated adsorption-pyrolysis method. The resulted HSC composite was characterized and employed as adsorbent for the removal of negatively charged arsenate (As(V)), positively charged Pb(II), and the organic pollutant methylene blue (MB) from aqueous solutions in batch experiments. The effects of solution pH, contact time, initial concentration, temperature, and ionic strength on As(V), Pb(II) and MB adsorption were investigated. HSC was composed of nano-size MgO flakes and nanotube-like carbon sponge. Hybridization significantly improved As(V), Pb(II) and methylene blue (MB) adsorption when compared with the material without hybridization. The maximum As(V), Pb(II) and MB adsorption capacities obtained from Langmuir model were 157 mg/g, 103 mg/g and 297 mg/g, respectively. As(V) adsorption onto HSC was best fit by the pseudo-second-order model, and Pb(II) and MB with the intraparticle diffusion model. Increased temperature and ionic strength decreased Pb(II) and MB adsorption onto HSC more than As(V). Further FT-IR, XRD and XPS analysis demonstrated that the removal of As(V) by HSC was mainly dominated by surface deposition of MgHAsO4 and Mg(H2AsO4)2 crystals on the HSC composite, while carbon π-π* transition and carbon π-electron played key roles in Pb(II) and MB adsorption. The interaction of Pb(II) with carbon matrix carboxylate was also evident. Overall, MgO hybridization improves the preparation of the nanotube-like carbon sponge composite and provides a potential agricultual residue-based adsorbent for As(V), Pb(II) and MB removal.

Removal of arsenate and dichromate ions from different aqueous media by amine based p(TAEA-co-GDE) microgels.

In this work, microgels based on tris(2-aminoethyl) amine (TAEA) and glycerol diglycidyl ether (GDE) via simple microemulsion polymerization was prepared as p(TAEA-co-GDE) microgels were used as adsorbent for removal of dichromate (Cr (VI)) and arsenate (As (V)) ions from different aqueous environments. The p(TAEA-co-GDE) microgels were demonstrated very efficient adsorption capacity for Cr (VI), and As (V) that are 164.98 mg/g, and 123.64 mg/g from distilled (DI) water, respectively. The effect of the medium pH on the adsorption capacity of p(TAEA-co-GDE) microgels for Cr (VI) and As (V) ions were investigated. The maximum adsorption capacity was obtained at pH 4.0 for both ions with maximum adsorbed amounts of 160.62, and 98.72 mg/g, respectively. In addition, the microgels were also shown moderate adsorption capacity for Cr (VI) and As (V) from other water sources; tap water with 115.18 mg/g and 82.86 mg/g, sea water with 64.24 mg/g and 46.88 mg/g and creek water with 73.52 mg/g and 59.33 mg/g, respectively. Moreover, the increase in adsorbent dose from 0.025 to 0.125 g enhanced % adsorption of Cr (VI) from 54.13 to 98.03, and As (V) from % 26.72-98.70, respectively. For the adsorption process Langmuir and Freundlich adsorption isotherms were applied and found that Langmuir adsorption isotherm with R2 value of 0.99 for both the metal ions are suitable. Moreover, the experimental adsorption capacities of Cr (VI) and As (V) were found very close to the theoretical values calculated from Langmuir adsorption isotherm. More importantly, the microgels were made magnetic responsive to recover them easily from adsorption medium for reuse studies by applying external magnetic field with little decrease in adsorption capacity. Additionally, reusability of p(TAEA-co-GDE) microgels was also evaluated for adsorption of Cr (VI) and As (V) from DI water.

pH modulates arsenic toxicity in Bacillus licheniformis DAS-2.

The toxic characteristics of arsenic species, As(V) and As(III) result in ecological risks. Arsenic tolerant bacterium was isolated and identified as the Bacillus licheniformis DAS-2 through 16SrDNA sequencing. B. licheniformis DAS-2 was efficient to tolerate and remove both the As(V)[MIC 8mM] and As(III)[MIC 6mM] from the growth medium. The potential for the removal/uptake of arsenic from the 3, 5 and 7mM As(V) enriched growth media was 100%, 60% and 35% respectively and from the 1, 3 and 5mM As(III) enrichment it was 100%, 99% and 58% respectively at neutral pH. 80% of uptake As(V) was reduced to As(III) in 3mM As(V) enrichment which was gradually decreased to only 17% at 7mM As(V) enrichment at neutral pH. The arsenic toxicity in B. licheniformis DAS-2 was found modulated by pH and was examined through alteration in growth, uptake/removal, reduction and measurement of chemical toxicity.

Biosorption of As(V) onto dried alligator weed root: role of metal (hydro) oxides.

The present work investigates the adsorption of As(V) onto the dried powder of alligator weed root as bio-sorbent, using acid pre-treated alligator weed root powder as the reference. The isotherm study suggested there is a favorable As(V) adsorption happened on the AWR surface. The batch adsorption experimental results indicated that the ionic strength has little impact on the adsorption, while the solution pH has a significant effect on the adsorption with apparent inhibition appearing in both extreme acidic and alkaline pH region. In addition, the properties of the biosorbent were characterized by various techniques including SEM-EDS, FT-IR, and ICP detection. The analysis results suggested that the metals including Mn, Fe, and Al enrich over the alligator weed root surface in the morphology of metal (hydro) oxide. Based on the nature of the biosorbent and As(V) besides the adsorption performance, the metal (hydro) oxides over biosorbent surface is suggested as the essential role to drive the adsorption. With the metal (hydro) oxides denuded in the pre-treatment, the biosorbent loses its adsorption capability for As(V) totally.

Removal of arsenic from water using manganese (III) oxide: Adsorption of As(III) and As(V).

Removal of arsenic from water was evaluated with manganese (III) oxide (Mn2O3) as adsorbent. Adsorption of As(III) and As(V) onto Mn2O3 was favorable according to the Langmuir and Freundlich adsorption equilibrium equations, while chemisorption of arsenic occurred according to the Dubinin-Radushkevich equation. Adsorption parameters from the Langmuir, Freundlich, and Temkin equations showed a greater adsorption and removal of As(III) than As(V) by Mn2O3. Maximum removal of As(III) and As(V) occurred at pH 3-9 and at pH 2, respectively, while removal of As(V) in the pH range of 6-9 was 93% (pH 6) to 61% (pH 9) of the maximum removal. Zeta potential measurements for Mn2O3 in As(III) was likely converted to As(V) solutions indicated that As(III) was likely converted to As(V) on the Mn2O3 surface at pH 3-9. Overall, the effective Mn2O3 sorbent rapidly removed As(III) and As(V) from water in the pH range of 6-9 for natural waters.

Multiple cysteine residues are necessary for sorting and transport activity of the arsenite permease Acr3p from Saccharomyces cerevisiae.

The yeast transporter Acr3p is a low affinity As(III)/H(+) and Sb(III)/H(+) antiporter located in the plasma membrane. It has been shown for bacterial Acr3 proteins that just a single cysteine residue, which is located in the middle of the fourth transmembrane region and conserved in all members of the Acr3 family, is essential for As(III) transport activity. Here, we report a systematic mutational analysis of all nine cysteine residues present in the Saccharomyces cerevisiae Acr3p. We found that mutagenesis of highly conserved Cys151 resulted in a complete loss of metalloid transport function. In addition, lack of Cys90 and Cys169, which are conserved in eukaryotic members of Acr3 family, impaired Acr3p trafficking to the plasma membrane and greatly reduced As(III) efflux, respectively. Mutagenesis of five other cysteines in Acr3p resulted in moderate reduction of As(III) transport capacities and sorting perturbations. Our data suggest that interaction of As(III) with multiple thiol groups in the yeast Acr3p may facilitate As(III) translocation across the plasma membrane.

Equilibrium and kinetics studies on As(V) and Sb(V) removal by Fe2+ -doped Mg-Al layered double hydroxides.

Mg-Al layered double hydroxides (Mg-Al LDHs) doped with Fe(2+) adsorbed As(V) [Formula: see text] and Sb(V) [Formula: see text] from an aqueous solution through anion exchange with Cl(-) intercalated in the LDH interlayer. Fe(2+)-doped Mg-Al LDH exhibited superior As(V) removal compared with Mg-Al LDH. The oxidation of Fe(2+) doped in the Mg-Al LDH host layer to Fe(3+) increased the positive layer charge of the LDH, thus increasing the anion-uptake capacity owing to stronger electrostatic attractive force between the positively charged layer and the anion. However, Fe(2+)-doped Mg-Al LDH was not superior to Mg-Al LDH in terms of Sb(V) removal. This was attributed to the preferential intercalation of OH(-) over [Formula: see text] . The As(V) and Sb(V) removal by LDH followed Langmuir-type adsorption, which proceeded via a pseudo-first-order reaction. The equilibrium and kinetics studies confirm that the adsorption of As(V) and Sb(V) by Fe(2+)-doped Mg-Al LDH was the result of chemical adsorption, involving the anion exchange of [Formula: see text] and [Formula: see text] with the intercalated Cl(-).