An hypoxia-tolerant flatfish: consequences of sustained stress on the slender sole Lyopsetta exilis (Pleuronectidae) in the context of a changing ocean.

Slender sole Lyopsetta exilis is an abundant groundfish on the continental shelf and inner waters of British Columbia, Canada, where it reaches a maximum standard length of 44 cm. Benthic image surveys coupled with oxygen measurements in Saanich Inlet document a dense population in bottom conditions near anoxia (0.03 ml l-1 oxygen) where diel migrating zooplankton intersect the bottom; we confirm this species is a planktivore, which limits its depth range to the base of the migration layer. In a comparison with slender sole from a nearby well-oxygenated habitat, several probable effects of living in severe hypoxia emerge: both sexes are significantly smaller in Saanich and the sex ratio is male-skewed. Otoliths from the Saanich fish were difficult to read due to many checks, but both sexes were smaller at age with the largest female (20 cm) from the hypoxia zone registering 17 years. Hypoxia appears to have a direct consequence on growth despite good food supply in this productive basin. Hyperventilation, a low metabolic rate and a very low critical oxygen tension help this fish regulate oxygen uptake in severely hypoxic conditions; it will be particularly resilient as the incidence of hypoxia increases on the continental shelf. Data from small-mesh bottom-trawl surveys over four decades reveal an increase in mean annual catch per unit effort in southern regions of the province, including the outer shelf and the Strait of Georgia. The California Cooperative Oceanic Fisheries Investigations (CalCOFI) ichthyoplankton database records a general decline in fish larvae on the Oregon-California shelf since 1990, but slender sole larvae are increasing there, as they are in the Strait of Georgia. We project that the slender sole will gain relative benefits in the future warming, deoxygenated northeast Pacific Ocean.

Megafaunal variation in the abyssal landscape of the Clarion Clipperton Zone.

The potential for imminent polymetallic nodule mining in the Clarion Clipperton Fracture Zone (CCZ) has attracted considerable scientific and public attention. This concern stems from both the extremely large seafloor areas that may be impacted by mining, and the very limited knowledge of the fauna and ecology of this region. The environmental factors regulating seafloor ecology are still very poorly understood. In this study, we focus on megafaunal ecology in the proposed conservation zone 'Area of Particular Environmental Interest 6' (study area centred 17°16'N, 122°55'W). We employ bathymetric data to objectively define three landscape types in the area (a level bottom Flat, an elevated Ridge, a depressed Trough; water depth 3950-4250 m) that are characteristic of the wider CCZ. We use direct seabed sampling to characterise the sedimentary environment in each landscape, detecting no statistically significant differences in particle size distributions or organic matter content. Additional seafloor characteristics and data on both the metazoan and xenophyophore components of the megafauna were derived by extensive photographic survey from an autonomous underwater vehicle. Image data revealed that there were statistically significant differences in seafloor cover by nodules and in the occurrence of other hard substrata habitat between landscapes. Statistically significant differences in megafauna standing stock, functional structuring, diversity, and faunal composition were detected between landscapes. The Flat and Ridge areas exhibited a significantly higher standing stock and a distinct assemblage composition compared to the Trough. Geomorphological variations, presumably regulating local bottom water flows and the occurrence of nodule and xenophyophore test substrata, between study areas may be the mechanism driving these assemblage differences. We also used these data to assess the influence of sampling unit size on the estimation of ecological parameters. We discuss these results in the contexts of regional benthic ecology and the appropriate management of potential mining activities in the CCZ and elsewhere in the deep ocean.

Cryptic diversity, geographical endemism and allopolyploidy in NE Pacific seaweeds.

BACKGROUND: Molecular markers are revealing a much more diverse and evolutionarily complex picture of marine biodiversity than previously anticipated. Cryptic and/or endemic marine species are continually being found throughout the world oceans, predominantly in inconspicuous tropical groups but also in larger, canopy-forming taxa from well studied temperate regions. Interspecific hybridization has also been found to be prevalent in many marine groups, for instance within dense congeneric assemblages, with introgressive gene-flow being the most common outcome. Here, using a congeneric phylogeographic approach, we investigated two monotypic and geographically complementary sister genera of north-east Pacific intertidal seaweeds (Hesperophycus and Pelvetiopsis), for which preliminary molecular tests revealed unexpected conflicts consistent with unrecognized cryptic diversity and hybridization. RESULTS: The three recovered mtDNA clades did not match a priori species delimitations. H. californicus was congruent, whereas widespread P. limitata encompassed two additional narrow-endemic species from California - P. arborescens (here genetically confirmed) and P. hybrida sp. nov. The congruence between the genotypic clusters and the mtDNA clades was absolute. Fixed heterozygosity was apparent in a high proportion of loci in P. limitata and P. hybrida, with genetic analyses showing that the latter was composed of both H. californicus and P. arborescens genomes. All four inferred species could be distinguished based on their general morphology. CONCLUSIONS: This study confirmed additional diversity and reticulation within NE Pacific Hesperophycus/Pelvetiopsis, including the validity of the much endangered, modern climatic relict P. arborescens, and the identification of a new, stable allopolyploid species (P. hybrida) with clearly discernable ancestry (♀ H. californicus x ♂ P. arborescens), morphology, and geographical distribution. Allopolyploid speciation is otherwise completely unknown in brown seaweeds, and its unique occurrence within this genus (P. limitata possibly representing a second example) remains enigmatic. The taxonomic separation of Hesperophycus and Pelvetiopsis is not supported and the genera should be synonymized; we retain only the latter. The transitional coastline between Point Conception and Monterey Bay represented a diversity hotspot for the genus and the likely sites of extraordinary evolutionary events of allopolyploid speciation at sympatric range contact zones. This study pinpoints how much diversity (and evolutionary processes) potentially remains undiscovered even on a conspicuous seaweed genus from the well-studied Californian intertidal shores let alone in other, less studied marine groups and regions/depths.

Estimating the Distribution of Harvested Estuarine Bivalves with Natural-History-Based Habitat Suitability Models.

Habitat suitability models are useful to estimate the potential distribution of a species of interest, particularly in the case of infaunal bivalves. Sampling for these bivalves is time- and cost-intensive, which is increasingly difficult for organizations or agencies that are limited by personnel and funds. Consequently, we developed a framework to identify suitable bivalve habitat in estuaries (FISBHE) - a habitat suitability index (HSI) modeling framework for NE Pacific estuaries that was parameterized with published natural-hi story information and existing habitat datasets, without requiring extensive field sampling of bivalves. Spatially explicit, rule-based habitat suitability models were constructed in a GIS for five species of bay-clams (Clinocardium nuttallii, My a arenaria, Tresus capax, Saxidomus gigantea, and Leukoma staminea) that are popular targets for recreational and commercial harvest in estuaries of the U.S. Pacific Northwest. Habitat rasters were produced for Yaquina and Tillamook estuaries (Oregon, USA) using environmental data (bathymetric depth, sediment % silt-clay, wet-season salinity, and burrowing shrimp presence/absence) from multiple studies (1953-2015). These habitat rasters then served as inputs in the final model which produced HSI classes ranging from 0-4 (lowest to highest suitability), dependent upon the number of habitat variables that fell within the sensitivity limits for each species of bay-clam. The models were tested with validation analyses and bay-clam occurrence data (reported in benthic community studies, 1996-2012) within each HSI class; logistic regression and Kendall's correlation coefficient both showed correspondence between predicted HSI classes and bay-clam presence/absence. Results also showed that the greatest presence probabilities occurred within habitats of highest predicted suitability, with the exception of M. arenaria in Tillamook Bay. The advantage of FISBHE is that disparate, independent sets of existing data are sufficient to parameterize the models, as well as produce and validate maps of habitat suitability. This approach can be transferred to data-poor systems with modest investment, which can be useful for prioritizing estuarine land-use decisions and could be used to estimate the vulnerability of this valued ecosystem good to changes in habitat quality and distribution.

A new Culeolus species (Ascidiacea) from the NE Pacific, California.

The paper describes Culeolus barryi n. sp. (Ascidiacea: Pyuridae), a species photographically documented in situ and collected in the northeast Pacific, off the coast of California (USA). This species of stalked tunicate inhabits deepwater (1200 m) and is characterized by numerous gonads on each side of the body. It is most similar to C. nadejdae from the Sea of Okhotsk and C. sluiteri from Aleutian Islands. A brief overview of all Culeolus species is provided.

Size and dynamics of microplastic in gastrointestinal tracts of Northern Fulmars (Fulmarus glacialis) and Sooty Shearwaters (Ardenna grisea).

We found microplastic in 89.5% of 143 Northern Fulmars from 2008 to 2013 and 64% of 25 Sooty Shearwaters in 2011-2012 that were found dead or stranded on Oregon and Washington beaches. Average plastic loads were 19.5 pieces and 0.461g for fulmars and 13.3 pieces and 0.335g for shearwaters. Pre-manufactured plastic pellets accounted for 8.5% of fulmar and 33% of shearwater plastic pieces. In both species, plastic in proventriculi averaged 2-3mm larger in greatest dimension than in ventriculi. Intestinal plastic in fulmars averaged 1mm less in greatest dimension than ventricular plastic. There was no significant reduction in pieces or mass of plastic in 33 fulmars held for a median of seven days in a plastic-free environment. Three fulmars that survived to be released from rehabilitation regurgitated plastic, which provided an alternative outlet for elimination of plastic and requires reassessment of the dynamics of plastic in seabird gastrointestinal tracts.

CHIHARAEA AND YAMADAIA (CORALLINALES, RHODOPHYTA) REPRESENT REDUCED AND RECENTLY DERIVED ARTICULATED CORALLINE MORPHOLOGIES(1).

Phycologists have hypothesized that the diminutive fronds produced by species in the genera Chiharaea and Yamadaia, which are composed of comparatively few genicula and intergenicula, represent morphological intermediates in the evolution of articulated corallines from crustose ancestors. We test this "intermediate frond hypothesis" by comparing rbcL sequences from the generitype species Chiharaea bodegensis and Yamadaia melobesioides to sequences from other coralline genera. We demonstrate that Chiharaea includes two other NE Pacific species, Arthrocardia silvae and Yamadaia americana. Chiharaea species are characterized morphologically by inflated intergenicula and axial conceptacles with apical or acentric pores. Although relationships among the three species are unresolved, Chiharaea bodegensis, C. americana comb. nov., and C. silvae comb. nov. are distinguished from one another by DNA sequences, morphology, habitat, and biogeography. Chiharaea occurs together with Alatocladia, Bossiella, Calliarthron, and Serraticardia macmillanii in a strongly supported clade of nearly endemic north Pacific articulated coralline genera and species that have evolved relatively recently compared to other articulated corallines. In contrast, NW Pacific Yamadaia melobesioides belongs in a clade with Corallina officinalis, the generitype species of Corallina, and therefore we reduce Yamadia to a synonym of Corallina and propose Corallina melobesioides comb. nov. We reject the 'intermediate frond hypothesis' and conclude that Chiharaea and Yamadaia are recently derived taxa that evolved from articulated coralline ancestors and represent a reduction in the number of genicula and intergenicula.