Alizarin: Prospects and sustainability for food safety and quality monitoring applications.

Active and intelligent food packaging has emerged to ensure food safety, quality, or spoilage monitoring and extend the shelf life of food. The development of intelligent packaging has accelerated significantly in recent years with a focus on monitoring changes in the quality of packaged products in real-time throughout the food supply chain. As one of the popular natural colorants, alizarin has attracted much consideration due to its excellent functional properties and quality to color change under varying pH. Alizarin is an efficient and cost-effective biomaterial with numerous biological features such as antioxidant, antibacterial, non-cytotoxic, and antitumor. This review focuses on an in-depth summary and prospects for alizarin as a natural and safe colorant that has the potential to be incorporated into intelligent packaging to track the freshness of packaged foodstuffs. The use of alizarin as an intelligent packaging agent shows huge potential for the application of food packaging and brings it one step closer to real-time monitoring of food quality throughout the supply chain. Finally, various limitations and future requirements are discussed to underscore the importance of developing alizarin-based intelligent functional food packaging systems.

A pH dependent Raman and surface enhanced Raman spectroscopic studies of citrazinic acid aided by theoretical calculations.

A pH dependent normal Raman scattering (NRS) and surface enhanced Raman scattering (SERS) spectral patterns of citrazinic acid (CZA), a biologically important molecule, have been investigated. The acid, with different pKa values (~4 and ~11) for the two different functional groups (-COOH and -OH groups), shows interesting range of color changes (yellow at pH~14 and brown at pH~2) with the variation in solution pH. Thus, depending upon the pH of the medium, CZA molecule can exist in various protonated and/or deprotonated forms. Here we have prescribed the existence different possible forms of CZA at different pH (Forms "C", "H" and "Dprot" at pH~14 and Forms "A", "D", and "P" at pH~2 respectively). The NRS spectra of these solutions and their respective SERS spectra over gold nanoparticles were recorded. The spectra clearly differ in their spectral profiles. For example the SERS spectra recorded with the CZA solution at pH~2 shows blue shift for different bands compared to its NRS window e.g. 406 to 450cm(-1), 616 to 632cm(-1), 1332 to 1343cm(-1) etc. Again, the most enhanced peak at ~1548cm(-1) in NRS while in the SERS window this appears at ~1580cm(-1). Similar observation was also made for CZA at pH~14. For example, the 423cm(-1) band in the NRS profile experience a blue shift and appears at ~447cm(-1) in the SERS spectrum as well as other bands at ~850, ~1067 and ~1214cm(-1) in the SERS window are markedly enhanced. It is also worth noting that the SERS spectra at the different pH also differ from each other. These spectral differences indicate the existence of various adsorptive forms of the CZA molecule depending upon the pH of the solution. Therefore based on the experimental findings we propose different possible molecular forms of CZA at different pH (acidic and alkaline) conditions. For example forms 'A', 'D' and 'P' existing in acidic pH (pH~2) and three other deprotonated forms 'C', 'H' and 'Dprot' in alkaline pH (pH~14). The DFT calculations for these prescribed model systems were also carried out to have a plausible understanding of their equilibrium geometries and the vibrational wavenumbers. An idea about the molecular orientation of the adsorbate over nanocolloidal gold substrate is also documented.