A distant relationship?-investigation of correlations between DNA isolated from backspatter traces recovered from firearms, wound profile characteristics, and shooting distance.

In molecular ballistics, where traces originating from the use of firearms against biological targets are investigated, "backspatter" traces are of particular importance. This biological material comprising blood and tissue from the victim is propelled back from the bullet entry site towards the direction of the shooter and can consolidate and persist on the inner and outer surfaces of the firearm, from where it can be collected and analyzed. Thus, a connection between the weapon and the victim can be established solely by molecular biological trace analysis. For the criminalistic investigation of gun-related crimes, the determination of the distance between the weapon and the victim can be of critical importance in reconstructing the circumstances of a crime. In this study, we investigated possible correlations between the shooting distance and the amount of backspatter in/on the used firearm. To this purpose, we employed a previously established skull model and performed shootings in triplicates from various distances up to 50 cm with two types of handguns (pistol and revolver). Backspatter was collected from various sampling locations, and DNA contents were quantified. A post-shooting wound channel evaluation was conducted by optical and radiological evaluation. The obtained DNA yields varied considerably between replicates from the same and from different distances. In contrast, apart from contact shots, no meaningful differences were observable in wound channel evaluations. In summary, no meaningful correlation between backspatter distribution and DNA yields, the shooting distance and the condition of the wound channel could be established.

Shooting accuracy in children's basketball: Do equipment dimensions influence static and dynamic performances?

The purpose of study was to explore the influence of equipment dimensions on shooting accuracy in children's basketball through the effects of (a) using basket height and ball size for children and adult, along with different shooting distances (Study 1) and (b) the applied 12-week shooting training with different ball sizes (Study 2). The participants were basketball players from the same club ages from 10 to 11 years and with at least one-year basketball training experiences. In Study 1, we recruited 22 children; while in Study 2 we selected 36 children in two experimental (practice with balls for adults and children) and one control group (no practice). Shooting accuracy was assessed through the static and dynamic trials. The obtained results showed that children equipment provided no acute advantages compared to the adult equipment in terms of shooting accuracy either in static or dynamic conditions. In addition, we found no benefits on shooting accuracy when compared the effects of applied shooting training either for practice with children's or adult's ball sizes. The present findings suggested that the applied manipulations of equipment for children and adult produce no differences in terms of static or dynamic shooting accuracy performances in young basketball players.

Gunshot residue on dark materials: a comparison between infrared photography and the use of an alternative light source.

The pattern of gunshot residue (GSR) includes important information about muzzle-target distance since a larger GSR distribution diameter indicates a larger shooting distance. GSR may not be visible to the naked eye when, for example, it is located on dark textiles. In such cases, further procedures need to be performed in order to visualize the pattern of GSR. Besides chemical procedures, an alternative light source or infrared photography can be utilized for non-destructive GSR visualization. In the work presented, these two techniques are compared based on shooting experiments using 26 different dark textiles. Within the range of the alternative light source, the use of a 440-nm light in combination with an orange-colored filter led to the best visualization of GSR in the form of fluorescent particles. Infrared photography, on the other hand, visualized GSR as dark particles, whereas-ideally-the dark textile reflected the infrared light and appeared bright. The comparison of both techniques revealed that the GSR distribution visualized by infrared photography was not identical to the GSR distribution visualized with 440-nm illumination in combination with an orange-colored filter. We concluded that infrared photography visualizes the inner powder soot zone, whereas illumination at 440 nm leads to fluorescence of the outer powder soot zone, which can be visualized using an orange-colored filter. Knowledge of this difference in visualization of the two powder soot zones is important for forensic practitioners assessing firing distances. In the literature, however, this difference is not noted as clearly.

A study of gunshot residue distribution for close-range shots with a silenced gun using optical and scanning electron microscopy, X-ray microanalysis and infrared spectroscopy.

Detailed physical and chemical analysis of gunshot residue deposited in the nearest vicinity of a submachine gun alone and with a sound suppressor was performed. The studies were inspired by recent shooting cases with the use of a firearm with a silencer and the need to estimate the shooting distance to human body naked and covered with clothing. A series of experiments were performed in the shooting range using a machine pistol and the appropriate ammunition cal. 7.65mm Browning. Targets were placed in the range of 0-30cm from the gun and covered either with white cotton fabric or a porcine skin that mocked people's clothing and the naked skin. Both the organic and inorganic residue were examined by means of optical microscopy, infrared spectrometry as well as scanning electron microscopy and energy dispersive X-ray spectrometry. The influence of factors, such as sound suppressor, shooting distance and the substrate type on the mechanism of particles spread and their availability for research was established and discussed.

Analysis of Crack Image Recognition Characteristics in Concrete Structures Depending on the Illumination and Image Acquisition Distance through Outdoor Experiments.

The effects of illumination and shooting distance on crack image recognition were investigated by examining cracks in images taken with a camera. In order to examine the effects, images of cracks in a concrete structure taken while varying the illumination and shooting distance in an outdoor environment were analyzed. The images were acquired at a daytime illumination of 52,000 lx and a night illumination of 13 lx. The crack specimen images produced for the experiment were taken by increasing the shooting distance from 5 m to 100 m in each illumination. On the basis of the analysis on the modulation transfer function (MTF) and contrast sensitivity of the crack images, the effects of illumination and shooting distance on the sharpness of the crack images were investigated. The minimum crack widths that can be identified under each illumination were analyzed using MTF10 and Weber contrast 0.1, respectively. It was found that as the shooting distance increases, the effects of illumination on crack recognition become greater.

Assessing the shooting distance of lead-free ammunition regardless of composition using Laser Induced Breakdown Spectroscopy.

At present, it is challenging to accurately determine firearm shooting distances in the case that lead-free ammunition is involved, largely because different manufacturers use different primer compositions. Laser-induced breakdown spectroscopy (LIBS) allows the simultaneous detection of multiple elements with high sensitivity and so may represent a solution to this problem. Previous studies have, in fact, demonstrated that LIBS can be used to determine shooting distances when working with gunshot residues from conventional ammunition based on scanning fabric surfaces. The present study confirms that the shooting distance can be ascertained using LIBS to detect copper originating from the ammunition casing and projectile but not the primer on fabric surfaces. This estimation can be performed regardless of the primer composition of lead-free ammunition. Key points: Evaluation of gunshot residue from lead-free ammunition using scanning electron microscopy-energy dispersive X-ray analysis indicated that 40% of the particles contained copper.The iForenLIBS system allowed the detection of copper-containing particles on fabric surfaces after firing at different distances with high sensitivity.Laser-induced breakdown spectroscopy can determine the shooting distance of lead-free ammunition through copper detection even in ammunition that does not used this element in the primer.This technique can generate density maps allowing the evaluation of short, medium, and long-range shooting distances.

The influence of a possible contamination of the victim's clothing on the estimation of shooting distance in mutual shooting incidents.

One of the tools for estimating shooting distance is examination of smokeless powder particle distribution on the target area. Components of the powder that are utilized for this purpose are nitrite anions. The traditional method for detecting nitrite anions mainly involves applying the Griess Test. A known-distance test firing is performed with shootings done at several distances from different targets. The color pattern corresponding to nitrite anions from the crime scene is then compared to the patterns obtained from known distances of the test firing. When a mutual shooting takes place at the crime scene, and a shooter also becomes a shootout victim, it is possible that when examining the shooter as a victim (i.e. target), additional nitrite-containing particles, resulting from his/her shooting, will be present on the shooter-victim clothing. This kind of addition may affect the estimation and practically give a shorter-distance estimation comparing to the actual distance. In this paper, an experimental setup was designed in order to understand if nitrite-containing particles were added to a victim as a consequence of him/her being also a shooter. All of the experiments were predominantly designed to try and minimize the effects of other influencing factors and variables in order to examine if the additions resulting from the firing action affect distance estimation. The experiments involved various types of pistols and distances. The results show that in such a scenario, there are marginal additions of nitrite signals on the victim's shirt. Although the forensic expert's final assessment was within the tolerance interval at all distances, caution should be exercised when attempting to estimate shooting distances in scenarios where the victim also shoots.

How may analysis of an inner layer of clothing affect the scene reconstruction in a shooting incident?

Shooting distances are utilized, among other forensic input, to position shooters and victims at the crime scene. Shooting distance estimation is done, mainly under the knowledge or assumption, that no intervening object has been present between the target and the muzzle. In the analysis of clothing items of victims that have been involved in a shootout, it is assumed that most of the marks and materials are left on the outer layers of clothing. The outer layers, not the inner layers, are the first objects the bullet meets. Consequently, undergarments are often disregarded for shooting distance estimation as they are not assumed to contain sufficient information in the form of marks or materials. In light of the above, it is the standard operating procedure in some forensic laboratories not to examine underwear. In this case report, the forensic examiners started by the implementation of standard operating procedures, thus examining only the outer layers of clothing of the victims. A thorough visual examination of the various clothing items led a forensic examiner to decide to extend the standard operating procedures, and try and examine an undergarment of one of the victims as well. The interpretation of examination products led to different scene reconstruction, one that may have led to a different interpretation of evidence. In this case, simply sticking to standard operating procedures might have led to less accurate crime scene reconstruction. This case report comes to stress the importance of thorough inspection of forensic exhibits, and scientific and critical thinking about different scenarios. Moreover, it demonstrates how the case-by-case approach in shooting distance estimation may add meaningful information, and eventually-bring us even closer to the truth.

Evaluating the effect of barrel length on pellet distribution patterns of sawn-off shotguns.

In recent years, sawn-off shotguns have been regularly encountered in firearm-related incidents. Shotgun represents a community threat due to their ability to be shortened in order to improve their concealability. In practice, forensic scientists and investigators may be asked to determine if a shotgun used in a particular event might have been shortened based on the pellet distribution. To investigate the effect of altering the barrel length on pellet distribution, five 12-gauge shotguns were progressively shortened. Each shotgun was assessed at six barrel lengths and a single discharge of No. 4 shot shotshell ammunition was fired at a fixed fabric target. The shotguns were positioned at six different distances from target and experiments conducted in triplicate. The fabric targets containing pellet pattern underwent data analysis to calculate the pellet distribution area. At short distances from target (≤ 1.0 m), the pellet penetration was observed as a single entry point as the pellets did not have a sufficient flight time to spread from each other. For distances of 2.5 m, 5 m and 10 m, pellet penetration patterns were observed and a quantitative analysis was performed on the samples containing pellet distribution patterns. At each of these distances, it was detected that there was a noticeable increase in pellet distribution area between the unaltered shotgun and altered barrel lengths for all shotguns. The distance from the target as well as the presence of a choke in the shotgun's barrel was found to have a greater impact on the pellet distribution than the barrel length.

Shot range estimation of shotgun grain-loaded cartridges.

Commercial grain-loaded cartridges for shotguns have been recently associated with injuries and fatalities. These cartridges are generally used as a scare gun for large animals, with a little probability of causing harm. Almost all grain-loaded cartridges contain one type of cereal grain, such as bulgur wheat, wheat or vetch seed, instead of lead pellets. Our study aimed to investigate the shot range estimation of grain-loaded cartridges and assess the variations from conventional lead pellet cartridges. In this study, eight different brands of grain-loaded cartridges were used. The inspections and measurements were made with cartridges that contain cereal grains, gunpowder and wads. Shots were made from 50, 100, 200, 300, 500cm with modified and improved cylinder chokes. Diameter of grain distribution patterns on targets were measured. These measurements were evaluated statistically considering brand, choke and distance variables. Both satellite entry holes and central entry holes were detected in all shots that were fired from 50cm distance. The smallest average diameter of grain distribution pattern between 8 different brands were measured as 14.3, 38.3, 58.5 and 83.4cm for 100, 200, 300 and 500cm respectively with modified choke. Gathered data from this study was compared with the studies conducted with conventional cartridges and differences were determined. It was detected that the grain-loaded cartridges showed much wider distribution on targets at the same shooting distance with the same and similar barrel lengths and choke values compared to conventional lead pellet containing cartridges.

Implementation and Optimization of the Sodium-Rhodizonate Method for Chemographic Shooting Distance Estimation.

In shooting incident investigations where it is important to estimate the shooting distance, the sodium-rhodizonate coloring method has been used for the visualization of GSR patterns extensively. This publication describes the optimization of this method by the use of a heated press for the transfer of GSR traces on garments, as well as variation of a number of other important process parameters. Our final aim for this project was to prepare a formal validation of the coloring method as prerequisite for its inclusion into the scope of ISO17025-certified methods in the GSR Laboratory. A number of important factors and process parameters were identified, such as buffer composition and pH, type of textile used as a substrate and resolution of the documentation of the results. Results were tested using a synthetic proficiency test. Our optimized method has been found to be a very effective technique for visualization of macroscopic GSR traces.

Multi-spectral imaging for the estimation of shooting distances.

Multispectral images of clothing targets shot at seven different distances (from 10 to 220cm) were recorded at 18 specific wavelengths in the 400-1000nm range to visualize the gunshot residue (GSR) pattern. Principal component analysis (PCA) showed that the use of violet-blue wavelengths (430, 450 and 470nm) provided the largest contrast between the GSR particles and the white cotton fabric. Then, the correlation between the amount of GSR particles on clothing targets and the shooting distance was studied. By selecting the blue frame of multispectral images (i.e. the blue frame in the red-green-blue (RGB) system which falls at 470nm), the amount of pixels containing GSR particles was accounted based on the intensity of pixels in that frame. Results demonstrated that the number of pixels containing GSR exponentially decreases with the shooting distance from 30 to 220cm following a particular exponential equation. However, the targets shot at the shortest distance (10cm) did not satisfy the above equation, probably due to the noticeable differences of the GSR-pattern of these targets (e.g. high presence of soot). Then, the equation was applied to validation samples to estimate the shooting distances, obtaining results with an error below 10%.

The effect of range and ammunition type on fracture patterns in porcine postcranial flat bones.

Pig half-carcasses were shot in scapulae, ribs and mandibles with either 0.243 hunting rifle using high velocity expanding ammunition (N = 30) or AK47 using full metal jacketed (FMJ) ammunition (N = 12) from a range of either 5 or 20 m. Fracture patterns related to distance of fire and ammunition type were compared on de-fleshed, macerated, and reconstructed bones. For expanding ammunition, location of fracture on ribs affected the resulting pattern. Scapulae shot from 5 m presented a comminuted pattern different from those shot from 20 m. Mandibles shot from 20 m showed a characteristic radiating pattern at entrance with the opposite ramus un-fractured; those shot from 5 m exhibited fractures to both rami. Using decision tree analysis provided accuracies of 93.8% for scapulae and 87.5% for mandibles. For FMJ, no distance dependent fracture differences were apparent in any bone. Decision tree analysis facilitated the interpretation of fracture patterns caused by projectile trauma.

Controversial Suicide Case Using a Submachine Gun with a Sound Suppressor-The Need of Team Work of Forensic Chemistry and Firearm Examiners.

Evidence materials in a presumed suicide case were studied by a firearm examiner and a forensic chemist. The victim's body with double gunshot wounding in his forehead, a machine gun in the sustained fire mode with a silencer, and four cartridge cases were found. Examinations of the evidence, the case file studies, and experiments dedicated to the case were carried out. Relationships between the placement of cartridge cases and the gun were established using a fast camera. The distributions of gunshot residues on the evidence materials and within the comparative gunshot patterns were studied by means of optical and electron microscopy, X-ray microanalysis, and infrared spectroscopy. The shooting distance was assessed to be 30 cm or more, whereas the greatest distance that could have been achieved by the victim himself was about 11-13 cm. The obtained results supported the version of homicide rather than suicide.

The use of unburned propellant powder for shooting-distance determination. Part I: Infrared luminescence.

Unburned propellant powder particles in gunshot residue (GSR) were detected at near infrared by optical excitation in the visible wavelength range. A series of ammunition (different brands and different manufacturers) was analyzed concerning the luminescence of their propellant. Shooting target samples with different shooting distances were produced on standard textile tissue and analyzed with this optical infrared inspection. The number of luminescent GSR particles per area was measured and curves with particle density vs. shooting distance were drawn. The method was applied on three ammunition types with different particle morphology shot with a pistol and one ammunition type shot with a revolver. The shooting series performed with the revolver showed a large particle density variation within the samples of identical shooting distances. In this case, the ratio of the amount of particles within the area around the bullet hole and within a ring with a defined distance from the bullet hole was calculated. These data resulted in measures with much lower standard deviations, which is a prove that the distribution pattern depends on the shooting distance and not on the amount of GSR particles. It has been shown, that imaging of target tissue with the aid of infrared luminescence is an easy, fast, reproducible and non-destructive method for shooting-distance determination.

Short range shooting distance estimation using variable pressure SEM images of the surroundings of bullet holes in textiles.

Modifications of cotton and polyester textiles due to shots fired at short range were analyzed with a variable pressure scanning electron microscope (VP-SEM). Different mechanisms of fiber rupture as a function of fiber type and shooting distance were detected, namely fusing, melting, scorching, and mechanical breakage. To estimate the firing distance, the approximately exponential decay of GSR coverage as a function of radial distance from the entrance hole was determined from image analysis, instead of relying on chemical analysis with EDX, which is problematic in the VP-SEM. A set of backscattered electron images, with sufficient magnification to discriminate micrometer wide GSR particles, was acquired at different radial distances from the entrance hole. The atomic number contrast between the GSR particles and the organic fibers allowed to find a robust procedure to segment the micrographs into binary images, in which the white pixel count was attributed to GSR coverage. The decrease of the white pixel count followed an exponential decay, and it was found that the reciprocal of the decay constant, obtained from the least-square fitting of the coverage data, showed a linear dependence on the shooting distance.

How far does it get?--The effect of shooting distance and type of firearm on the simultaneous analysis of DNA and RNA from backspatter recovered from inside and outside surfaces of firearms.

When a firearm projectile hits a biological target a spray of biological material (e.g. blood and tissue) is ejected from the entrance wound and propelled back into the direction of the firearm. This phenomenon has been termed 'backspatter' and if backspattered biological material reaches the firearm on its backward trajectory it may persist on and be recovered from the firearm's inside surfaces. Molecular genetic analysis of backspatter generated by contact shots and shots from very short distances has already been demonstrated to critically contribute to victim identification and the reconstruction of firearm-related crimes. It is not known, however, up to what shooting distance can backspatter be found on firearms' inside surfaces and what influence the weapon's type and caliber has on backspatter attributes (e.g. reach, amount and distribution). Therefore, the present pilot study investigated the effect of serval combinations of shooting distances and types of firearms and ammunitions on the analyzability of co-extracted DNA and micro-RNA in samples of backspatter collected from interior and exterior surfaces of the firearms after experimental shootings employing standardized ballistic models. We demonstrate the limiting effect of shooting distance and the type of firearm on the yield of nucleic acids recovered from backspatter and the success rates of forensic DNA profiling and RNA based body-fluid and organ tissue identification in experimental shootings.

Recent non-chemical approaches to estimate the shooting distance.

Shooting distance estimation offers useful information for the reconstruction of firearm related incidents. The muzzle to target distance is usually estimated by examining the bullet entrance hole and the gunshot residue pattern. To visualize the pattern the forensic analyst usually uses presumptive tests based on color chemical reactions that are applied using long and tedious proceedings. Due to the drawbacks of the chemical tests recent developments for shooting distance estimation not based on color chemical tests were described in the literature. The present review covers the approaches for shooting distance estimation published in the last 10 years considering two types of target, clothing and skin.

Methods for the Determination of Shooting Distance.

The review deals with scientific methods for determining shooting distance. The different procedures are explained, their advantages and disadvantages are discussed. The methods are divided into chemographical processes and spectroscopic techniques. Special emphasis is given to the methods proven to be especially useful in daily work, but methods used in basic research are briefly described as well. Furthermore, attention is paid to newly developed ammunitions. In addition, a survey is given on the chemical elements found in gunshot residences (GSR).