Alternatives Exert Higher Health Risks than Bisphenol A on Indo-Pacific Humpback Dolphins.

The detrimental effects of bisphenol (BP) exposure are a concern for vulnerable species, Indo-Pacific humpback dolphins (Sousa chinensis). To investigate the characteristics of BP profiles and their adverse impact on humpback dolphins, we assessed the concentrations of six BPs, including bisphenol A (BPA), bisphenol S (BPS), bisphenol F (BPF), bisphenol AF (BPAF), bisphenol B (BPB), and bisphenol P (BPP) in blubber (n = 26) and kidney (n = 12) of humpback dolphins stranded in the Pearl River Estuary, China. BPS accounted for the largest proportion of the total bisphenols (∑BPs) in blubber (55%) and kidney (69%). The concentration of ∑BP in blubber was significantly higher than that in the kidney and liver. The EC50 values of five BPA alternatives were lower than those of BPA in humpback dolphin skin fibroblasts (ScSF) and human skin fibroblasts (HSF). ScSF was more sensitive to BPS, BPAF, BPB, and BPP than HSF. The enrichment pathway of BPA was found to be associated with inflammation and immune dysregulation, while BPP and BPS demonstrated a preference for genotoxicity. BPA, BPP, and BPS, which had risk quotients (RQs) > 1, were found to contribute to subhealth and chronic disease in humpback dolphins. According to the EC50-based risk assessment, BPS poses a higher health risk than BPA for humpback dolphins. This study successfully evaluated the risks of bisphenols in rare and endangered cetacean cell lines using a noninvasive method. More in vivo and in field observations are necessary to know whether the BPA alternatives are likely to be regrettable substitutions.

Assessing the toxicity of contaminated soils via direct contact using a gas production bioassay of thiosulfate utilizing denitrifying bacteria (TUDB).

A direct contact bioassay of thiosulfate utilizing denitrifying bacteria (TUDB) based on inhibition of gas production was deployed to assess the toxicity of naturally contaminated field soils and soils artificially contaminated with heavy metals. Test procedure producing optimal conditions responsible for maximum gas production was 0.5 mL test culture, 1 g soil sample, 80 RPM, and 48 h reaction time. Similarly, the concentrations which generated a 50% reduction in gas production by TUDB for the tested heavy metals were 3.01 mg/kg Cr6+; 15.30 mg/kg Ni2+;15.50 mg/kg Cu2+;16.60 mg/kg Ag+; 20.60 mg/kg As3+; 32.80 mg/kg Hg2+; 54.70 mg/kg Cd2+; and 74.0 mg/kg Pb2+. Because soil toxicity is usually influenced by various physicochemical characteristics, ten reference soils were used to determine the toxicity threshold for evaluating the toxicity of naturally contaminated field soils. All eight contaminated soils were toxic to the TUDB bioassay because their levels of inhibition ranged between 72% and 100% and exceeded the determined toxicity threshold of 10%. Compared to other direct contact assays, the newly developed assay TUDB proved to be very robust, producing highly sensitive data while the different soil physicochemical properties exerted minimal influence on the gas production activity of TUDB. Additionally, the simplicity of the developed methodology coupled with the elimination of pretreatment procedures such as elutriation, and ability to perform generate sensitive data in turbid and highly colored samples makes it, cost-effective, and easily adaptable for the assessment of heavy metal and field contaminated soils when compared with other conventional assays which require sophisticated instrumentation and prolonged testing procedures and times.

A direct contact bioassay using immobilized microalgal balls to evaluate the toxicity of contaminated field soils.

The present study used a bioassay of immobilized microalgae (Chlorella vulgaris) via direct contact to assess the toxicity of eleven uncontaminated (reference) and five field contaminated soils with various physicochemical properties and contamination. Photosynthetic oxygen concentration in the headspace of the test kit by Chlorella vulgaris in the reference soils ranged between 12.93% and 14.80% and only 2.54%-7.14% in the contaminated soils, respectively. Inherent test variability (CVi) values ranged between 2.90% and 9.04%; variation due to soil natural properties (CVrs) ranged between 0.33% and 13.0%; and minimal detectable difference (MDD) values ranged from 4.69% to 11.6%. A computed toxicity threshold of 15% was established for microalgae soil toxicity tests based on calculations of the maximal tolerable inhibition (MTI). All contaminated soils were considered toxic to microalgae because their levels of inhibition ranged between 39.5% and 82.9%, exceeding the 15% toxicity threshold. It can be concluded that the elevated concentrations of heavy metals and organic contaminants in the contaminated soils induced the higher inhibitory levels. Overall, direct contact soil toxicity tests using immobilized microalgae provided coherent and repeatable data and can be utilized as a simple and suitable tool for the toxicity testing of contaminated field soils.

The toxicity of exogenous arsenic to soil-dwelling springtail Folsomia candida in relation to soil properties and aging time.

Arsenic (As) is a toxic metalloid, but studies on As toxicity to soil-dwelling springtails are fairly limited, and did not consider the effects of various soil properties and long aging time. To address this, the toxicity of As to model organism-Folsomia candida were evaluated in the laboratory studies. The results showed that compared to the soils aged only for 15 d, the concentrations inhibiting 50% reproduction (EC50) significantly increased by 1.3- to 2.0-fold in four soils aged for 150 d, the concentrations causing 50% mortality (LC50) were higher than the highest test concentration in the most soils. Furthermore, the aging effects correlated significantly with soil free Fe oxides contents. The toxicity of As differed in ten soils aged for 150 d, the LC50 were 320-> 1280 mg/kg in acute test and the EC50 were 67-580 mg/kg in chronic test. Regression analysis indicated that soil clay was the most important single factor predicting soil As toxicity to reproduction, explaining 89% of the variance in EC50 values. Soil pH, free Fe oxides and Al oxides could also well explain the toxicity variance (> 65%), indicating that As sorption was a key factor controlling its toxicity.

Quantitative differentiation of toxicity contributions and predicted global risk of fipronil and its transformation products to aquatic invertebrates.

Biotransformation often alters chemical toxicity, yet its impacts on risk assessment are hardly quantified due to the challenges in acquiring internal exposure-based thresholds for chemicals that are readily metabolizable. Here, we integrated toxic unit and toxicokinetics to quantitatively assess toxicity contributions and potential risk of both parent compound and transformation products (TPs) to aquatic organisms, using fipronil (FIP) as a representative toxicant. In aquatic invertebrates Chironomus dilutus and Hyalella azteca, approximately 90 % of FIP was transformed to fipronil sulfone (SUL). FIP and SUL exhibited similar intrinsic toxicity to these organisms, which was contrary to conventional perception that SUL was more toxic than FIP. However, biotransformation was still important in risk assessment because the TP had 10-fold slower depuration rate than FIP. The amphipod H. azteca was found to be as sensitive to FIPs as the insect C. dilutus, which was previously considered ten times more sensitive based on external thresholds. This discrepancy has led to overlooking the toxicity of FIP to H. azteca in regional risk assessments. Lastly, we predicted the lethal risk of FIPs in global surface water. When using external thresholds for prediction, FIPs in 3.4 % of the water samples were lethally toxic to H. azteca, and the percentage of water samples at risk increased to 14 % when internal thresholds were used and SUF dominated the risk. This study presents an improved method for quantifying aquatic risk of readily metabolized toxicants. Our findings underscore the urgency of considering TPs in water quality assessments, especially for sensitive species that are at risk in the environment.

Interactive effects of ammonium sulfate and lead on alfalfa in rare earth tailings: Physiological responses and toxicity thresholds.

Ion-adsorption rare earth ore contains significant levels of leaching agents and heavy metals, leading to substantial co-contamination. This presents significant challenges for ecological rehabilitation, yet there is limited understanding of the toxicity thresholds associated with the co-contamination of ammonium sulfate (AS) and lead (Pb) on pioneer plants. Here, we investigated the toxicity thresholds of various aspects of alfalfa, including growth, ultrastructural changes, metabolism, antioxidant system response, and Pb accumulation. The results indicated that the co-contamination of AS-Pb decreased the dry weight of shoot and root by 26 %-77 % and 18 %-92 %, respectively, leading to irregular root cell morphology and nucleus disintegration. The high concentration and combined exposures to AS and Pb induced oxidative stress on alfalfa, which stimulated the defense of the antioxidative system and resulted in an increase in proline levels and a decrease in soluble sugars. Structural equation modeling analysis and integrated biomarker response elucidated that the soluble sugars, proline, and POD were the key physiological indicators of alfalfa under stresses and indicated that co-exposure induced more severe oxidative stress in alfalfa. The toxicity thresholds under single exposure were 496 (EC5), 566 (EC10), 719 (EC25), 940 (EC50) mg kg-1 for AS and 505 (EC5), 539 (EC10), 605 (EC25), 678 (EC50) mg kg-1 for Pb. This study showed that AS-Pb pollution notably influenced plant growth performance and had negative impacts on the growth processes, metabolite levels, and the antioxidant system in plants. Our findings contribute to a theoretical foundation and research necessity for evaluating ecological risks in mining areas and assessing the suitability of ecological restoration strategies.

An assessment of the relation between metal contaminated sediment and freshwater mussel populations in the Big River, Missouri.

The Big River in southeast Missouri drains the largest historical lead mining area in the United States. Ongoing releases of metal contaminated sediments into this river are well documented and are suspected of suppressing freshwater mussel populations. We characterized the spatial extent of metal contaminated sediments and evaluated its relationship with mussel populations in the Big River. Mussels and sediments were collected at 34 sites with potential metal effects and 3 reference sites. Analysis of sediment samples showed that lead (Pb) and zinc (Zn) concentrations were 1.5 to 65 times greater than background concentrations in the reach extending 168 km downstream from Pb mining releases. Mussel abundance decreased acutely downstream from these releases where sediment Pb concentrations were highest and increased gradually as Pb sediment concentrations attenuated downstream. We compared current species richness with historical survey data from three reference rivers with similar physical habitat characteristics and human effects, but without Pb-contaminated sediment. Big River species richness was on average about one-half that expected based on reference stream populations and was 70-75 % lower in reaches with high median Pb concentrations. Sediment Zn and cadmium, and particularly Pb, had significant negative correlations with species richness and abundance. The association of sediment Pb concentrations with mussel community metrics in otherwise high-quality habitat indicates that Pb toxicity is likely responsible for depressed mussel populations observed within the Big River. We used concentration-response regressions of mussel density verses sediment Pb to determine that the Big River mussel community is adversely affected when sediment Pb concentrations are above 166 ppm, the concentration associated with 50 % decreases in mussel density. Based on this assessment of metals concentrations sediment and mussel fauna, our findings indicate that sediment in approximately 140 km of the Big River with suitable habitat has a toxic effect to mussels.

Soil ammonium (NH4+) toxicity thresholds for restoration grass species.

Ionic rare earth mining has resulted in large amounts of bare soils, and revegetation success plays an important role in mine site rehabilitation and environmental management. However, the mining soils still maintain high NH4+ concentrations that inhibit plant growth and NH4+ toxicity thresholds for restoration plants have not been established. Here we investigated the NH4+ toxicological effects and provided toxicity thresholds for grasses (Lolium perenne L. and Medicago sativa L.) commonly used in restoration. The results show that high NH4+ concentration not only reduces the plant biomass and soluble sugars in leaves but also increases the H2O2 and MDA content, and SOD, POD, and GPX activities in roots. The SOD activities and root biomass can be adopted as the most NH4+ sensitive biomarkers. Six ecotoxicological endpoints (root biomass, soluble sugars, proline, H2O2, MDA, and GSH) of ryegrass, eight ecotoxicological endpoints (root biomass, soluble sugars, proline, MDA, SOD, POD, GPX, and GSH) of alfalfa were selected to determine the threshold concentrations. The toxicity thresholds of NH4+ concentrations were proposed as 171.9 (EC5), 207.8 (EC10), 286.6 (EC25), 382.3 (EC50) mg kg-1 for ryegrass and 171.9 (EC5), 193.2 (EC10), 234.7 (EC25), 289.6 (EC50) mg kg-1 for alfalfa. The toxicity thresholds and the relation between plant physiological indicators and NH4+ concentrations can be used to assess the suitability of the investigated plants for ecological restoration strategies.

Ecological toxicity (ECx) of Pb and its prediction models in Chinese soils with different physiochemical properties.

The lack of toxicological data becomes the main bottleneck of ecological risk assessment of lead (Pb) in Chinese soils. The present study assessed Pb toxicity on three underground test endpoints (barley root elongation, earthworm avoidance response, and substrate-induced respiration (SIR) of microorganism) in 10 different soils. Hormetic dose-response induced by Pb was >118 % for earthworm avoidance response. EC10 and EC50 (the effective concentrations of Pb that inhibit 10 % or 50 % of endpoint bioactivity and also represents the toxicity threshold of Pb) after leaching increased by 0.32-8.73 times, and 1.02-3.75 times, respectively. Leaching factor (LF) prediction models indicated pH and cation exchange capacity (CEC) were the vital predictors for LF10 and LF50, explaining 60.6 % and 73.1 % of variations, respectively. SIR was one sensitive test endpoint for Pb toxicity, with the lowest of EC10 and EC50 values (from 373.7 to 1008.5 mg·kg-1, and from 837.1 to 2869.0 mg·kg-1, respectively). The best prediction models between ECx and soil properties is LogEC50 = 1.324Log(pH) + 0.423Log(CEC) + 1.742 (R2 = 0.761, p < 0.01). The results displayed significant implications for deriving ECx of Pb, and provided a scientific basis for soil ecological risk assessment of Pb.

Aging factor and its prediction models of chromium ecotoxicity in soils with various properties.

Aging of pollutants determines bioavailability and toxicity thresholds of environmental pollutants in soil. However, the ecotoxicity of chromium (Cr) rarely considers the effect of aging as well as soil properties. In order to explore the aging characteristics and establish their quantitative relationship with different soil properties, this study selected 7 soils with different properties through exogenous addition of Cr and determined its toxicity on barley root elongation. From 14d to 540d, EC10 and EC50 of barley root elongation ranged from 21.40 to 312.52 (mg·kg-1) and 50.15 to 883.88 (mg·kg-1) respectively. The hormesis appeared in the dose-response curve of acid soil as relative barley root elongation reached >110 % compared with the control. Extended aging time of Cr from 14d to 540d was associated with the attenuation of the toxicity of Cr, as the aging factor increased from 1.26 to 6.09 for EC50, from 0.88 to 4.98 for EC10. The prediction model of AFEC50 and soil properties is lg (AF360d) = 0.306lg Clay+0.026lg CEC + 0.240 (R2 = 0.872, P < 0.01). The results demonstrated that with the extension of aging time, the toxicity of Cr decreased at 360d and reached a slow reaction stage, after that soil OC, Clay and CEC could well explain the aging procedure of Cr (VI). These results are beneficial for risk assessment of Cr contaminated soils and establishment of a soil environmental quality criteria for Cr.

Subtoxic levels of some heavy metals cause differential root-shoot structure, morphology and auxins levels in Arabidopsis thaliana.

Contamination of soil by heavy metals severely affects plant growth and causes soil pollution. While effects on plant growth have been investigated for metals taken individually or in groups, less is known about their comparative effects. In this study Arabidopsis thaliana seedlings were grown for 14 days in Petri dishes containing medium contaminated by six common heavy metals (Hg, Cd, Pb, Cu, Ni and Zn), at the minimum concentrations defined as toxic by the most recent EU legislation on contamination of agricultural soils. (a) Root structure and morphology, (b) metal composition and translocation, and (c) the levels of indole-3-acetic acid (IAA) and indole-3-butyric acid (IBA) were analyzed. Metals accumulated more in roots than in shoots, with concentrations that differed by several orders of magnitude depending on the metal: Cd (ca. 700 × and ca. 450 × in roots and shoots, respectively), Hg (150 × , 80 × ), Ni (50 × , 20 × ), Cu (48 × , 20 × ), Zn (23 × , 6 × ), and Pb (9 × , 4 × ). Responses were significant for at least nine of the ten root parameters (with the exception of Hg), and five of the six shoot parameters (with the exception of Zn). Cu and Zn induced respectively the strongest responses in root hormonal (up to ca. 240% the control values for IBA, 190% for IAA) and structural parameters (up to 210% for main root length, 330% for total lateral root length, 220% for number of root tips, 600% for total root surface, and from 2.5° to 26.0° of root growth angle). Regarding the shoots, the largest changes occurred for shoot height (down to 60% for Ni), rosette diameter (down to 45% for Hg), leaf number (up to 230% for Zn) and IBA (up to 240% for Pb and Cu). A microscope analysis revealed that shape and conformation of root hairs were strongly inhibited after Cd exposure, and enhanced under Hg and Pb. The results could have positive applications such as for defining toxicity thresholds (in phytoremediation) and acceptable concentration levels (for policies) for some of the most common heavy metals in agricultural soils.

A direct contact bioassay using sulfur-oxidizing bacteria (SOB) for toxicity assessment of contaminated field soils.

In this study, 11 low/uncontaminated (including Lufa 2.2) and 9 contaminated field soils with varying geophysical and physicochemical characteristics were evaluated for toxicities based on oxygen consumption of sulfur-oxidizing bacteria (SOB). Oxygen consumption of the low/uncontaminated soils ranged between 7.9 mL and 9.5 mL, while contaminated soils ranged between 0.4 mL and 5.4 mL. Inherent test variability (CVi), variation due to soil natural properties (CVns) and minimal detectable difference (MDD) values ranged 1.2%-3.9%, 3.5%-16.9%, and 2.1%-4.3%, respectively. The toxicity threshold of 20% was established for soil toxicity based maximal tolerable inhibition (MTI). All the contaminated soils were found to be toxic and showed inhibition between 42% and 100% above the 20% threshold value. Increased proportions of clay and slit enhanced the of inhibitory effect of contaminants on SOB by reducing the oxygen consumption. Current study provides a suitable method for the rapid toxicity assessment of contaminated field soils with the advantages of ease of handling and rapidity without employing elutriates and sophisticated equipments and tools.

Toxicity of exogenous hexavalent chromium to soil-dwelling springtail Folsomia candida in relation to soil properties and aging time.

Chromium (Cr) is a well-known toxic metal, but studies on Cr toxicity to soil-dwelling springtails are fairly limited, and did not consider the effects of various soil properties and long aging time. To address this, the chronic toxicity of Cr(VI) to survival and reproduction of model organism-Folsomia candida were evaluated in the laboratory studies. The results showed that compared to the soils aged only for 2 and 21 d, the concentrations inhibiting 50% reproduction (EC50) significantly increased by 2.8-5.2 fold and 1.7-2.6 fold, the concentrations causing 50% mortality (LC50) were higher than the highest test concentration in four soils aged for 150 d. Furthermore, the aging effects correlated significantly with soil amorphous Fe oxides. The EC50 values of Cr significantly differed in ten soils aged for 150 d, ranging from 27 to 512 mg kg-1, which were associated with the variations in reduction and sorption capacity in different soils. Regression analysis indicated that soil clay was the most important single factor predicting soil Cr toxicity to reproduction, and the inclusion of cation exchange capacity in the clay regression could best explain the toxicity variance (87.2%). Additionally, soil pH, organic matter and amorphous Fe oxides could also well explain the toxicity variance (>55%).

Toxicity screening of soils from different mine areas--a contribution to track the sensitivity and variability of Arthrobacter globiformis assay.

This study used the Arthrobacter globiformis solid-contact test for assessing the quality of soils collected in areas subjected to past and present mine activities in Europe (uranium mine, Portugal) and North Africa (phosphogypsum pile, Tunisia; iron mine, Morocco). As to discriminate the influence of soils natural variability from the effect of contaminants, toxicity thresholds were derived for this test, based on the dataset of each study area. Furthermore, the test sensitivity and variability was also evaluated. As a result, soils that inhibited A. globiformis dehydrogenase activity above 45% or 50% relatively to the control, were considered to be toxic. Despite the soil metal content determined, the properties of soils seemed to influence dehydrogenase activity. Overall, the contact test provided a coherent outcome comparing to other more time-consuming and effort-demanding ecotoxicological assays. Our results strengthened the feasibility and ecological relevance of this assay, which variability was quite reduced hence suggesting its potential integration within the test battery of tier 1 of soil risk assessment schemes.