Single live birth derived from conjoined oocytes using laser-cutting technique: a case report.

The finding of conjoined oocytes is a rare occurrence that accounts for only 0.3% of all human retrieved oocytes. This phenomenon is quite different from that of a traditional single oocyte emanating from one follicle, and may result in dizygotic twins and mosaicism. Given the insufficient evidence on how to approach conjoined oocytes, their fate is variable among different in vitro fertilization (IVF) centres. In this observational report, we propose a new protocol for the use of these conjoined oocytes using intracytoplasmic sperm injection (ICSI), laser-cutting technique and next-generation sequencing (NGS). The first case report demonstrates that conjoined oocytes can penetrate their shared zona pellucida (ZP) at Day 6. The second case is that of a 25-year-old female patient who underwent a successful embryo transfer cycle after removal of one oocyte in which a pair of conjoined human oocytes underwent ICSI, laser-cutting separation and NGS testing. The patient achieved pregnancy and gave birth to single healthy female originally derived from conjoined oocytes. This case provided a means through which normal pregnancy may be achieved from conjoined oocytes using laser-cutting separation techniques. The protocol described may be especially beneficial to patients with a limited number of oocytes.

Green and Integrated Wearable Electrochemical Sensor for Chloride Detection in Sweat.

Wearable sensors for sweat biomarkers can provide facile analyte capability and monitoring for several diseases. In this work, a green wearable sensor for sweat absorption and chloride sensing is presented. In order to produce a sustainable device, polylactic acid (PLA) was used for both the substrate and the sweat absorption pad fabrication. The sensor material for chloride detection consisted of silver-based reference, working, and counter electrodes obtained from upcycled compact discs. The PLA substrates were prepared by thermal bonding of PLA sheets obtained via a flat die extruder, prototyped in single functional layers via CO2 laser cutting, and bonded via hot-press. The effect of cold plasma treatment on the transparency and bonding strength of PLA sheets was investigated. The PLA membrane, to act as a sweat absorption pad, was directly deposited onto the membrane holder layer by means of an electrolyte-assisted electrospinning technique. The membrane adhesion capacity was investigated by indentation tests in both dry and wet modes. The integrated device made of PLA and silver-based electrodes was used to quantify chloride ions. The calibration tests revealed that the proposed sensor platform could quantify chloride ions in a sensitive and reproducible way. The chloride ions were also quantified in a real sweat sample collected from a healthy volunteer. Therefore, we demonstrated the feasibility of a green and integrated sweat sensor that can be applied directly on human skin to quantify chloride ions.

Low-Cost Laser-Acoustic PVC Identification System Based on a Simple Neural Network.

Desktop laser cutters are an affordable and flexible rapid-prototyping tool, but some materials cannot be safely processed. Among them is polyvinyl chloride (PVC), which users usually cannot distinguish from other, unproblematic plastics. Therefore, an identification system for PVC applicable in a low-cost laser cutter has been developed. For the first time, this approach makes use of the laser-ablative sound generated by a low-power laser diode. Using a capacitor microphone, a preprocessing algorithm and a very simple neural network, black PVC could be detected with absolute reliability under ideal conditions. With ambient noise, the accuracy dropped to 80%. A different color of the material did not influence the accuracy to detect PVC, but a susceptibility of the method against a color change was found for other materials. The ablation characteristics for different materials were recorded using a fast-framing camera to get a better insight into the mechanisms behind the investigated process. Although there is still potential for improvements, the presented method was found to be promising to enhance the safety of future desktop laser cutters.

Laser Cut Interruption Detection from Small Images by Using Convolutional Neural Network.

In this publication, we use a small convolutional neural network to detect cut interruptions during laser cutting from single images of a high-speed camera. A camera takes images without additional illumination at a resolution of 32 × 64 pixels from cutting steel sheets of varying thicknesses with different laser parameter combinations and classifies them into cuts and cut interruptions. After a short learning period of five epochs on a certain sheet thickness, the images are classified with a low error rate of 0.05%. The use of color images reveals slight advantages with lower error rates over greyscale images, since, during cut interruptions, the image color changes towards blue. A training set on all sheet thicknesses in one network results in tests error rates below 0.1%. This low error rate and the short calculation time of 120 µs on a standard CPU makes the system industrially applicable.

Simultaneous Burr and Cut Interruption Detection during Laser Cutting with Neural Networks.

In this contribution, we compare basic neural networks with convolutional neural networks for cut failure classification during fiber laser cutting. The experiments are performed by cutting thin electrical sheets with a 500 W single-mode fiber laser while taking coaxial camera images for the classification. The quality is grouped in the categories good cut, cuts with burr formation and cut interruptions. Indeed, our results reveal that both cut failures can be detected with one system. Independent of the neural network design and size, a minimum classification accuracy of 92.8% is achieved, which could be increased with more complex networks to 95.8%. Thus, convolutional neural networks reveal a slight performance advantage over basic neural networks, which yet is accompanied by a higher calculation time, which nevertheless is still below 2 ms. In a separated examination, cut interruptions can be detected with much higher accuracy as compared to burr formation. Overall, the results reveal the possibility to detect burr formations and cut interruptions during laser cutting simultaneously with high accuracy, as being desirable for industrial applications.

Changes in corneal biomechanics during small-incision lenticule extraction (SMILE) and femtosecond-assisted laser in situ keratomileusis (FS-LASIK).

Observe the influence of femtosecond laser cutting on corneal biomechanics during small-incision lenticule extraction (SMILE) or femtosecond-assisted laser in situ keratomileusis (FS-LASIK) and assess the biomechanical changes following the operation. Prospective, non-randomized study. A total of 80 eyes from 80 patients were treated with either SMILE or FS-LASIK. Parameters of inverse concave radius, deformation amplitude ratio 2 mm or 1 mm (DA ratio 2 mm or 1 mm), highest concavity radius (HC radius), biomechanically corrected intraocular pressure (bIOP), and central corneal thickness (CCT) measured by Corvis ST II were recorded at 1 day preoperatively, immediately after the lenticule or flap creation, during subsequent lenticule extraction or excimer laser ablation, and during follow-up at 1 week, 1 month and 3 months postoperatively. After lenticule creation, the DA ratio 2 mm or 1 mm was bigger (p < 0.05), meanwhile, CCT was thicker (p < 0.05) than with flap creation. Partial parameters changed significantly after lenticule creation or flap creation, and all parameters changed significantly after tissue removal. All parameters showed no significant differences between the two groups (p > 0.05) after operation. The variations in bIOP (ΔbIOP) after operation were significantly less than those in Δnon-contact IOP (p < 0.001) in two groups. Femtosecond laser cutting during lenticule creation has a greater impact on corneal biomechanics than flap creation. Both the femtosecond laser cutting and removal of tissue degrade corneal biomechanics; however, these effects may be predominantly a result of tissue removal. SMILE and FS-LASIK have no differences in corneal biomechanics when the same CCT is consumed. bIOP is more reliable after the operation, but further study is essential.

Characterization of air contaminants emitted during laser cutting of carbon fiber-reinforced composite materials.

The emission of ultrafine carbonaceous particles during the laser cutting of fiber-reinforced polymer (CFRP) composite materials was investigated. The study was based on characterization of air contaminants emitted during laser cutting of an epoxy-based CFRP material with respect to particle size distribution, particle morphology, and chemical composition. Results indicate that about 90% of the total particulate mass is present as fine particulate matter with an aerodynamic cut-off diameter of 0.25 µm, and considerable amounts of ultrafine carbonaceous particulate matter dominated by organic carbon are emitted during high-power laser cutting of CFRP.

Fabrication of Dissolvable Microneedle Patches Using an Innovative Laser-Cut Mould Design to Shortlist Potentially Transungual Delivery Systems: In Vitro Evaluation.

There has been a great interest towards transungual delivery systems due to limited drug penetration for the treatment of nail diseases. More important, antifungal oral medicaments used may cause serious side effects including liver damage. Therefore, we propose non-oral dissolvable microneedle (MN) patch to strike the poor permeability of the nail. We report the design of MN patch mould using a laser-cutting machine and solvent casting of several hydrophilic polymers to fabricate these MN patches. Formulations were evaluated for their in vitro release and penetration properties and selected based on physical characterization for compatibility (differential scanning calorimetry (DSC) and powder X-ray diffraction (PXRD)), dimension repeatability and drug content uniformity. A 72-array of cone-shaped MN patch mould was successfully constructed on polymethylmethacrylate sheets. Interval and frequency of laser exposure were pivotal to determine the needle sharpness, attained unexpectedly at a low level of circa 30 µm. F1 platform of polyvinyl alcohol, kollicoat IR®, ethylene glycol and gelatin showed circa 74% penetration of methylhydroxy-4-benzoate (F1(A)) over 24 h, whereas F2 (same as F1-A with the addition of poloxamer 338) resulted in an almost 42% of this drug retention in the bovine hoof (24 h). Both formulations are likely to be useful for onychomycosis treatment. F1 polymers also afford enhanced permeability (almost 73.5% after 24 h) of terbinafine hydrochloride into the hoof (F1(B)). However, F3 (chitosan, gelatin and ethylene glycol) presents the prospect of developing MN patch for this drug with almost complete hoof penetration (circa 96.3% after 24 h). All medicated formulations have shown similar mechanical properties after ageing for 1 year under dry conditions.

Cutting processability of metal-ion-containing cellulose nanofibril films by continuous wave laser.

Understanding the cutting processability of cellulose nanofibril (CNF) films by continuous wave laser is important for precise shape processing that closely follows the design pattern. In this study, laser cutting of films made of surface-carboxylated CNFs with various counterionic species was performed to explore the factors that control the cutting processability. The cut width and the thermally affected width are mainly controlled by the laser irradiation energy per unit length. The processed cross section is tapered and rises above the film thickness. NMR analysis suggests that the pyrolysates contain water-soluble cello-oligosaccharides, the molecular weight of which varies with the type of CNF film. We consequently demonstrated that the COOH-type CNF film is preferable to the COONa-type CNF film for reducing the coloration residue and for processing the film into a shape that best follows the designed processing pattern.

Effects of Using Laser Technology for Cutting Polymer Films.

In connection with the need to obtain a properly made and cut material and the appearance of the surface layer, new manufacturing technologies were used for tests, namely the laser cutting technology. This article describes the laboratory stand built for the purpose of research, as well as the possibility of using laser cutting on several sample materials (polymer films), together with an indication of the results obtained. The idea was to elaborate on the cutting technology that will be proper for manufacturing the desired type of spacers for ion-exchange membranes separating while maintaining the required level of product quality and chemical purity. The latter criterion was the basic one, due to the scope of use of the manufactured elements. This article also describes the problem encountered during the construction of the stand or during the research. The last part of this article describes the further steps of the research that will be carried out in the future along with a discussion and summary of the research performed. It is important from the point of view of the development of production technology, but also because of the characteristics of materials for the production of surface layers and coatings resistant to mechanical or thermal wear used in industry. The introduction of innovative solutions is also aimed at studying the improvement of the economics of the production of materials that are significant, in particular, for small- and medium-sized enterprises.

A New Silicon Mold Process for Polydimethylsiloxane Microchannels.

As an alternative to SU-8 soft lithography, a new silicon mold process of fabricating PDMS microchannel chips was proposed. A picosecond laser is used to cut through a 550 µm thick silicon wafer and generate the original microchannel pattern with a 50 µm minimum feature size. This single-crystal silicon pattern, with the edge debris caused by laser cutting being trimmed off by a KOH solution and with the protection field oxide layer being removed by BOE afterwards, firmly resided on a glass substrate through the anodic bonding technique. Four-inch wafers with microchannel patterns as the PDMS mold cores were successfully bonded on Pyrex 7740 or Eagle XG glass substrates for the follow-up PDMS molding/demolding process. This new maskless process does not need a photolithography facility, but the laser cutting service must be provided by professional off-campus companies. One PDMS microchannel chip for particle separation was shown as an example of what can be achieved when using this new process.

Light-Responsive Soft Robot Integrating Actuation and Function Based on Laser Cutting.

Soft robots with good deformability and adaptability have important prospects in the bionics and intelligence field. However, current research into soft robots is primarily limited to the study of actuators and ignores the integrated use of functional devices and actuators. To enrich the functions of soft robots and expand their application fields, it is necessary to integrate various functional electronic devices into soft robots to perform diverse functions during dynamic deformation. Therefore, this paper discusses methods and strategies to manufacture optical stimuli-responsive soft actuators and integrate them into functional devices for soft robots. Specifically, laser cutting allows us to fabricate an optically responsive actuator structure, e.g., the curling direction can be controlled by adjusting the direction of the cutting line. Actuators with different bending curvatures, including nonbending, can be obtained by adjusting the cutting depth, cutting width, and the spacing of the cutting line, which makes it easy to obtain a folded structure. Thus, various actuators with complex shape patterns can be obtained. In addition, we demonstrate a fabrication scheme for a worm-like soft robot integrated with functional devices (LEDs are used in this paper). The local nonbending design provides an asymmetric structure that provides driving power and avoids damage to the functional circuit caused by the large deformation during movement. The integration of drive and function provides a new path for the application of soft robots in the intelligence and bionics field.

Research and Application Progress of Laser-Processing Technology in Diamond Micro-Fabrication.

Laser-processing technology has been widely used in the ultra-precision machining of diamond materials. It has the advantages of high precision and high efficiency, especially in the field of super-hard materials and high-precision parts manufacturing. This paper explains the fundamental principles of diamond laser processing, introduces the interaction mechanisms between various types of lasers and diamond materials, focuses on analyzing the current development status of various modes of laser processing of diamond, briefly discusses the relevant applications in diamond cutting, micro-hole forming, and micro-groove machining, etc., and finally discusses the issues, challenges, and potential future advancements of laser technology in the field of diamond processing at this point.

Double-Sided Tape in Microfluidics: A Cost-Effective Method in Device Fabrication.

The demand for easy-to-use, affordable, accessible, and reliable technology is increasing in biological, chemical, and medical research. Microfluidic devices have the potential to meet these standards by offering cost-effective, highly sensitive, and highly specific diagnostic tests with rapid performance and minimal sample volumes. Traditional microfluidic device fabrication methods, such as photolithography and soft lithography, are time-consuming and require specialized equipment and expertise, making them costly and less accessible to researchers and clinicians and limiting the applicability and potential of microfluidic devices. To address this, researchers have turned to using new low-cost materials, such as double-sided tape for microfluidic device fabrication, which offers simple and low-cost processes. The innovation of low-cost and easy-to-make microfluidic devices improves the potential for more devices to be transitioned from laboratories to commercialized products found in stores, offices, and homes. This review serves as a comprehensive summary of the growing interest in and use of double-sided tape-based microfluidic devices in the last 20 years. It discusses the advantages of using double-sided tape, the fabrication techniques used to create and bond microfluidic devices, and the limitations of this approach in certain applications.

The Advancement of Waterjet-Guided Laser Cutting System for Enhanced Surface Quality in AISI 1020 Steel Sheets.

This study investigates the effects of a water-guided laser on the cutting performance of AISI 1020 steel sheets of various thicknesses by comparing the results with respect to a conventional laser. For this purpose, a novel nozzle design has been devised enabling the delivery of laser beams to the workpiece conventionally as well as through water guidance. Diverging from prior literature, a fiber laser is used with a high wavelength and a laser power output of 1 kW. Experiments are conducted on steel sheets with thicknesses ranging from 1.5 mm to 3 mm using three different cutting speeds and laser power levels. Analysis focuses on assessing surface roughness, burr formation and heat effects on the cut surfaces for both conventional and waterjet-guided cutting. Surface roughness is evaluated by using a 3D profilometer and cut surfaces are examined through SEM imaging. The results showed that the waterjet-guided laser system greatly reduced surface roughness and minimized problems associated with traditional laser cutting such as kerf, dross adherence and thermal damage. The study revealed that cutting speed had a greater effect on surface roughness reduction than laser power, with the most noticeable differences occurring in thinner sheets. Furthermore, the investigation suggests that the waterjet-guided laser cutting system demonstrates superior performance relative to conventional methods, particularly in surface quality.

Design and Analysis of 5-DOF Compact Electromagnetic Levitation Actuator for Lens Control of Laser Cutting Machine.

In laser beam processing, the angle or offset between the auxiliary gas and the laser beam axis have been proved to be two new process optimization parameters for improving cutting speed and quality. However, a traditional electromechanical actuator cannot achieve high-speed and high-precision motion control with a compact structure. This paper proposes a magnetic levitation actuator which could realize the 5-DOF motion control of a lens using six groups of differential electromagnets. At first, the nonlinear characteristic of a magnetic driving force was analyzed by establishing an analytical model and finite element calculation. Then, the dynamic model of the magnetic levitation actuator was established using the Taylor series. And the mathematical relationship between the detected distance and five-degree-of-freedom was determined. Next, the centralized control system based on PID control was designed. Finally, a driving test was carried out to verify the five-degrees-of-freedom motion of the proposed electromagnetic levitation actuator. The results show it can achieve a stable levitation and precision positioning with a desired command motion. It also proves that the proposed magnetic levitation actuator has the potential application in an off-axis laser cutting machine tool.

Experimental Modeling, Statistical Analysis, and Optimization of the Laser-Cutting Process of Hardox 400 Steel.

Fiber laser cutting machines are widely used in industry for cutting various sheet metals. Hardox steel is widely used in the construction of machinery and equipment that are subjected to wear and impact due to its anti-wear properties and good impact resistance. In this experimental study, the effect of input parameters including laser output power (LOP), laser-cutting speed (LCS), and focal point position (FPP) of fiber laser on the surface roughness and kerf width of Hardox 400 steel sheets are studied. In addition, the optimization of input parameters to achieve the desired surface roughness and kerf width are investigated and analyzed using the response surface methodology (RSM). The experiments are performed using a 4 kW fiber laser-cutting machine and the output results including surface roughness and kerf width are measured using roughness meters and optical microscope. The results of the analysis of variance (ANOVA) for surface roughness and kerf width show that the FPP and LCS are the most significant process parameters affecting the surface roughness and kerf width. With a positive focal point, the surface roughness decreases while the kerf width increases. With increasing the laser-cutting speed, both the surface roughness and kerf width decrease.

Impacts of H2O2, SARM1 inhibition, and high NAm concentrations on Huntington's disease laser-induced degeneration.

Axonal degeneration is a key component of neurodegenerative diseases such as Huntington's disease (HD), Alzheimer's disease, and amyotrophic lateral sclerosis. Nicotinamide, an NAD+ precursor, has long since been implicated in axonal protection and reduction of degeneration. However, studies on nicotinamide (NAm) supplementation in humans indicate that NAm has no protective effect. Sterile alpha and toll/interleukin receptor motif-containing protein 1 (SARM1) regulates several cell responses to axonal damage and has been implicated in promoting neuronal degeneration. SARM1 inhibition seems to result in protection from neuronal degeneration while hydrogen peroxide has been implicated in oxidative stress and axonal degeneration. The effects of laser-induced axonal damage in wild-type and HD dorsal root ganglion cells treated with NAm, hydrogen peroxide (H2O2), and SARM1 inhibitor DSRM-3716 were investigated and the cell body width, axon width, axonal strength, and axon shrinkage post laser-induced injury were measured.

Process Analysis and Topography Evaluation for Monocrystalline Silicon Laser Cutting-Off.

Due to the characteristics of high brittleness and low fracture toughness of monocrystalline silicon, its high precision and high-quality cutting have great challenges. Aiming at the urgent need of wafer cutting with high efficiency, this paper investigates the influence law of different laser processes on the size of the groove and the machining affected zone of laser cutting. The experimental results show that when laser cutting monocrystalline silicon, in addition to generating a groove, there will also be a machining affected zone on both sides of the groove and the size of both will directly affect the cutting quality. After wiping the thermal products generated by cutting on the material surface, the machining affected zone and the recast layer in the cutting seam can basically be eliminated to generate a wider cutting seam and the surface after wiping is basically the same as that before cutting. Increasing the laser cutting times will increase the width of the material's machining affected zone and the groove width after chip removal. When the cutting times are less than 80, increasing the cutting times will increase the groove width at the same time; but, after the cutting times exceed 80, the groove width abruptly decreases and then slowly increases. In addition, the lower the laser scanning speed, the larger the width of the material's machining affected zone and the width of the groove after chip removal. The increase in laser frequency will increase the crack width and the crack width after chip removal but decrease the machining affected zone width. The laser pulse width has a certain effect on the cutting quality but it does not show regularity. When the pulse width is 0.3 ns the cutting quality is the best and when the pulse width is 0.15 ns the cutting quality is the worst.

Minimizing Thermal Damage During Thulium Laser-Assisted Partial Arytenoidectomy: Pulsed Versus Continuous Cutting in an Ex-Vivo Calf Model.

OBJECTIVES: The 2 µm-wavelength thulium laser is an effective cutter during partial arytenoidectomy, but thermal trauma can damage adjacent laryngeal tissue. Pulsing laser energy may reduce trauma when compared to continuous-wave cutting. This study measured temperature changes, thermal trauma, and time to complete partial arytenoidectomy, with and without pulsing, in an ex-vivo calf model. METHODS: Tissue temperature and time to complete a trans-cartilaginous cut were measured during partial arytenoidectomy on ex-vivo calf vocal folds (N = 24) using a thulium laser in continuous-wave (CW, N = 12) and pulsed-wave (PW, N = 12) modes. Energy was 5 W for CW and PW cuts; pulse-widths were 250, 500, and 750 ms. Thermal damage was analyzed histologically by measuring the depth of lactate dehydrogenase (LDH) inactivation perpendicular to the laser-cut edge at the vocal process. Paired t-tests compared CW and PW modes. RESULTS: Change in temperature was lower using CW (6.5°C) compared to PW modes (250 ms = 18°; 500 ms = 16°; 750 = 19°; P < .05). Trans-cartilaginous cuts were completed faster using CW (37 seconds) compared to PW (250 ms = 136 seconds; 500 ms = 61 seconds; 750 = 44 seconds; P < .05), and both modes delivered the same total Joules. The average depth of LDH depletion (thermal damage) was similar for all cuts. CONCLUSIONS: 1. Thulium laser cuts in continuous-mode unexpectedly produced less tissue heating yet created similar thermal damage than pulsed-mode cuts during simulated partial arytenoidectomy. 2. Trans-cartilaginous cuts were completed significantly faster in continuous-mode as compared to pulsed-mode cutting. 3. Pulsing the thulium laser does not minimize thermal damage compared to continuous wave cutting during thulium laser-assisted partial arytenoidectomy.