RESUMEN
Understanding the mechanisms underlying diapause formation is crucial for gaining insight into adaptive survival strategies across various species. In this study, we aimed to uncover the pivotal role of temperature and food availability in regulating diapausing podocyst formation in the jellyfish Aurelia coerulea. Furthermore, we explored the cellular and molecular basis of diapause formation using single-cell RNA sequencing. Our results showed cell-type-specific transcriptional landscapes during podocyst formation, which were underscored by the activation of specific transcription factors and signalling pathways. In addition, we found that the heat shock protein-coding genes HSC70 and HSP90a potentially act as hub genes that regulate podocyst formation. Finally, we mapped the single-cell atlas of diapausing podocysts and identified cell types involved in metabolism, environmental sensing, defence and development that may collectively contribute to the long-term survival and regulated excystment of diapausing podocysts. Taken together, the findings of this study provide novel insights into the molecular mechanisms that regulate diapause formation and contributes to a better understanding of adaptive survival strategies in a variety of ecological contexts.
Asunto(s)
Diapausa , Escifozoos , Animales , Escifozoos/genética , Temperatura , Diapausa/genéticaRESUMEN
The scyphozoan genus Aurelia is one of the main contributors causing jellyfish blooms in global coastal waters. The population and reproduction of polyps are important factors to the outbreak of jellyfish. In this study, the effects of different salinity on the survival and asexual reproduction of Aurelia coerulea polyps were explored, which were cultured at the high (21 â) and low temperature (12 â), respectively. The results showed that more than 90% polyps could survive in the salinity range of 15-40 at the high temperature, with a range of salinity for budding being 20-32 and an optimum value of 28. The polyps were able to produce podocysts to tolerate low (≤15) and high (≥36) salinity conditions. At the low temperature, the survival rates of polyps were more than 90% in the salinity range of 20-40. The polyps could produce more buds in the salinity range of 20-32, with an optimum of 28. The salinity range of 20-40 was suitable for strobilation, with an optimum range of 28-32. It is concluded that the polyps of Aurelia coerulea can tolerate wide salinity variations, and that the salinity in a certain range has no significant influence on the asexual reproduction of polyps.