Greenhouse gas emissions from liquid dairy manure: Prediction and mitigation.

The handling and use of manure on livestock farms contributes to emissions of the greenhouse gases (GHG) CH4 and N2O, especially with liquid manure management. Dairy farms are diverse with respect to manure management, with practices ranging from daily spreading to long-term storage for more efficient recycling of manure nutrients for crop production. Opportunities for GHG mitigation will depend on the baseline situation with respect to handling and storage, and therefore prediction and mitigation at the farm level requires a dynamic description of housing systems and storage conditions, and use of treatment technologies. Also, effects of treatment and handling on the properties of field-applied manure must be taken into account. Storage conditions and manure composition importantly define carbon and nitrogen transformations, and the resulting emissions of CH4 and N2O, as well as CO2 and NH3, which are all important for the GHG balance. Currently, inventories for CH4 and N2O emissions from manure are based on emission factors for a limited number of production systems, together with average annual temperature, but the inherent uncertainty of this approach is a barrier toward prediction and mitigation. Although more representative emission factors may be determined at country level, this is both challenging and costly, and effects of management changes for GHG mitigation are not easily quantified. An empirical model of CH4 emissions during storage is discussed that is based on daily time steps, and a parameterization based on measurements. A distinction between emissions from manure in barns and outside storage facilities is important for assessing effects of treatment technologies, such as anaerobic digestion, where only posttreatment emissions are affected. Upon field application, manure and soil together define the equilibrium distribution of labile carbon and nitrogen between bulk soil and manure hotspots. This introduces heterogeneity with respect to potential for N2O emissions, which is not represented in existing prediction models. Manure treatment and management options for GHG mitigation are discussed with emphasis on effects on manure volatile solids and N availability. Anaerobic digestion and acidification represent treatment technologies that are relevant for GHG mitigation on dairy farms.

Evaluation of reproductive toxicity in male rats treated with novel synthesized ruthenium(II) and gold(I)-NHC complexes.

In this study, we aimed to determine the reproductive toxicity in rat induced by ruthenium(II)-NHC (Ru(II)) and gold(I)-NHC (Au(I)) complexes that have anticarcinogenic effects. For this purpose, 35 Sprague-Dawley rats were randomly divided into 5 equal groups. In control group, rats treated with saline, Ru(II), and Au(I) complexes were intraperitoneally given high (10 mg/kg) and low (5 mg/kg) doses to rats via a one-time administration. The animals were sacrificed, and testis tissues were taken on Day 10 of the drug administration for the determination of the biochemical, histopathological, spermatological, and hormonal parameters. It was determined that treatment group that was subjected to treatment using both Ru(II) and Au(I) complexes significantly caused oxidative, histopathological, spermatological, and hormonal damage compared to control group. However, the sexual and accessory organ weight did not significantly change when compared to control. In addition, it was shown that Au(I) treatment generally caused more adverse effects than Ru(II) treatment in a dose-dependent manner. In conclusion, when these synthesized compounds are used for the treatment of cancer, they could cause toxic effects on male reproductive system and lead to infertility. However, Ru(II) complex is a more preferable option in cancer treatment, particularly in terms of user safety.

Nutrient content, in vitro ruminal fermentation characteristics and methane reduction potential of tropical tannin-containing leaves.

BACKGROUND: Plant tannins as rumen modifiers are better than chemicals or antibiotic-based modifiers since these compounds are natural products which are environmentally friendly and therefore have a better acceptance with regard to feed safety issues. Tropical plants containing phenols such as tannins were found to suppress or eliminate protozoa from the rumen and reduce methane and ammonia production. The screening of these plants is an important step in the identification of new compounds and feed additives which might contribute to mitigate rumen methanogenesis. The present study was carried out to determine the efficacy of tannins from tropical tree leaves for their methane reduction properties. RESULTS: Activity of tannins, as represented by the increase in gas volume with the addition of polyethylene glycol (PEG)-6000 as a tannin binder (tannin bioassay) was highest in Ficus bengalensis (555%), followed by Azardirachta indica (78.5%). PEG addition did not alter (P > 0.05) methane percentage in Ficus racemosa, Glyricidia maculata, Leucena leucocephala, Morus alba and Semaroba glauca, confirming that tannins in these samples did not affect methanogenesis. The increase (P < 0.05) in protozoa population with PEG was maximal in Ficus religiosa (50), followed by Moringa oleifera (31.2), Azardirachta indica (29.9) and Semaroba glauca (27.5). There was no change (P > 0.05) in the protozoa population in Autocarpus integrifolia, Ficus bengalensis, Jatropha curcus, Morus alba and Sesbania grandiflora, demonstrating that methane reduction observed in these samples per se was not due to defaunation effect of the tannin. The increase in total volatile fatty acid concentration in samples with PEG ranged from 0.6% to > 70%. The highest increase (%) in NH(3)-N was recorded in Azardirachta indica (67.4), followed by Ficus mysoriensis (35.7) and Semaroba glauca (32.6) leaves, reflecting strong protein binding properties of tannin. CONCLUSION: The results of our study established that in vitro methanogenesis was not essentially related to the density of protozoa population. Tropical tree leaves containing tannins such as Autocarpus integrifolia, Jatropha curcus and Sesbania grandiflora have the potential to suppress methanogenesis. Therefore tannins contained in these plants could be of interest in the development of new additives in ruminant nutrition.

Landfills, complexity and biogas risk assessment.

The purpose of this paper is to introduce the context of complexity in landfills as a conceptual framework for biogas risk assessment procedures. The paper suggests that there is an endogenous non-predictability to landfills' accidents and sudden events, which is created by the iterative process of building a landfill and the emerging complexity that characterizes it. Following the view of complexity a conceptual framework for a new biogas risk assessment model is proposed.

Building flux capacity: Citizen scientists increase resolution of soil greenhouse gas fluxes.

Though citizen science programs have been broadly successful in diverse scientific fields, their adoption has lagged in some disciplines, including soil science and ecosystem ecology. Collaborations with citizen scientists may be viewed as a conundrum in these disciplines, which often require substantial labor and technical experience; citizen scientists could improve sampling capacity but may reduce sample quality or require training and oversight prior to and while performing specialized tasks. To demonstrate the feasibility of incorporating citizen scientists into soil biogeochemistry research, we conducted a proof-of-concept study in high-elevation meadows of the Sierra Nevada in California. A collaboration between university researchers and citizen scientists allowed us to assess spatial and diel patterns of soil greenhouse gas (GHG) fluxes with an intensity and frequency that would otherwise be beyond the capacity of a typical research laboratory. This collaboration with citizen scientists increased our sampling intensity by over 700% while only doubling the sampling error relative to that of full-time researchers. With training and support from project scientists, citizen scientists collected data that demonstrate spatial independence of carbon dioxide, methane, and nitrous oxide at scales between 1 m and 175 m. Additionally, we found a lack of temporal variation over a 24-h period for all three GHGs. Citizen scientists participating in this one-day event reported levels of satisfaction commensurate with longer-term, immersive campaigns. The place-based event also proved an effective tool for teaching intangible concepts of soil biogeochemistry and promoting local conservation. Despite perceived barriers to entry, this study demonstrates the mutual benefits of citizen science collaborations in soil science and ecosystem ecology, encouraging adoption by disciplines that have been slow to take advantage of such collaborations. Short-term, local citizen science events can provide meaningful experiences for area residents and teach global biogeochemical cycles in a place-based context.

[Emissions from dairy industry and the influence of herd management]. / Emissionen aus der Milchrinderhaltung und ihre Beeinflussung durch das Herdenmanagement.

The purpose of this paper is to identify specific emission-reduction opportunities in dairy herds arising from aspects of useful herd management with the potential to reduce emissions, which are within the scope of veterinary activities. In future, it might be one of a veterinarian's advisory capacities to deal with the aspect of climate and environmental protection in animal husbandry. The models involved are similar to those of the national agricultural emission inventory. They allow quantifying the impacts of improved animal health, extended productive lifespan and grazing of an entire dairy herd (cows, calves, heifers and bulls) on emissions from the herd itself, in addition to those originating from the entire production chain, including provision of primary energy, water, feed production and processing. Ammonia emissions are the main focus. The reductions achieved here are not huge, though noticeable. They do not create extra costs. As can be shown, improved animal health and welfare are also environmentally beneficial. The reduction of greenhouse gas and air pollutant (eutrophying and acidifying gases and particles) emissions is an acknowledged political goal. If Germany wants to achieve the emission ceilings it has agreed to, agriculture will have to contribute. Planning will have to precede action if agriculture is itself to keep control of the processes.

Case-control study of colon cancer and drinking water trihalomethanes in Wisconsin.

A case-control study was conducted to test the hypothesis that chronic ingestion of trihalomethanes (THMs), occurring as chlorination byproducts in drinking water, carries a risk of colon cancer. Lifetime residential and water source histories and information on water-drinking habits, diet, sociodemographics, medical and occupation histories, lifestyle and other factors were obtained by questionnaire from a statewide sample of newly-diagnosed colon cancer cases (N = 347), controls with cancer of other sites (N = 639) and general population controls (N = 611). Since no data on past THM levels exists, it was necessary to devise a scheme to generate THM estimates for all Wisconsin water sources. For this, a statistical model based on quantitative THM measures and routinely-recorded data taken at 81 municipal water facilities was used in conjunction with individual residential histories to estimate lifetime and period-specific THM exposure for each case and control. Logistic regression was used to estimate odds ratios adjusted for age, sex and urban living, for colon cancer and THM exposure. The study results indicate that THM in Wisconsin drinking water does not pose a significant colon cancer risk. Odds ratios for exposure to the middle and highest category of lifetime cumulative THM were 1.05 (95% Cl = 0.66-1.68) and 0.93(95%Cl = 0.55-1.57) respectively, relative to the cancer control group, and 1.10 (95%Cl = 0.68-1.78) and 0.73 (95% Cl = 0.44-1.21) respectively, relative to the general population controls.(ABSTRACT TRUNCATED AT 250 WORDS)