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1.
Allergol Immunopathol (Madr) ; 46(6): 607-611, 2018.
Artículo en Inglés | MEDLINE | ID: mdl-29456036

RESUMEN

Food protein-induced enterocolitis syndrome (FPIES) is a non IgE-mediated gastrointestinal food allergic disorder. Some diagnostic criteria have been published for acute FPIES. Of course, they are not all the same, so the clinician must choose which ones to adopt for his/her clinical practice. We present here a brief review of these criteria and, through two clinical cases, show how the choice of one or the other can change the diagnostic destiny of a child with suspect FPIES.


Asunto(s)
Alérgenos/inmunología , Proteínas Dietéticas del Huevo/inmunología , Proteínas de Peces en la Dieta/inmunología , Hipersensibilidad a los Alimentos/diagnóstico , Tracto Gastrointestinal/patología , Enfermedad Aguda , Preescolar , Dietoterapia , Enterocolitis , Femenino , Hipersensibilidad a los Alimentos/dietoterapia , Humanos , Tolerancia Inmunológica , Lactante , Masculino , Síndrome
5.
Food Chem ; 302: 125333, 2020 Jan 01.
Artículo en Inglés | MEDLINE | ID: mdl-31416005

RESUMEN

This study was performed to determine Parvalbumin (PV), a well-known fish allergenic protein, digestion kinetics and immunoreactivity of digestion products with Immunoglobulin G/Immunoglobulin E recognition to understand its allergic potential with or without lipid emulsion process. PV was subjected to simulated gastrointestinal digestion in emulsified condition. Digestion kinetics of the protein was analysed by electrophoresis, IgG/IgE binding ability by immunoblotting and indirect ELISA. Lipid emulsion significantly (p < 0.01) reduced the degree of PV hydrolysis by 52.10% for gastric digestion. Immune fragments of gastric digestion were detectable for 90-120 min longer in emulsified condition showing resistance. Consequently, lipid emulsion decreased the digestive ability of PV in stomach, increasing resistance to gastrointestinal digestion by pepsin proteases. It also altered IgG/IgE binding ability of digestion products, thereby indicating that PV with lipid emulsion was resistant to digestion and possessed increased IgE binding ability resulting in higher risk of allergy among sensitized individuals.


Asunto(s)
Alérgenos/farmacocinética , Emulsiones/farmacocinética , Proteínas de Peces en la Dieta/farmacocinética , Hipersensibilidad a los Alimentos/inmunología , Parvalbúminas/farmacocinética , Alérgenos/inmunología , Alérgenos/metabolismo , Animales , Digestión , Emulsiones/química , Ensayo de Inmunoadsorción Enzimática , Femenino , Proteínas de Peces en la Dieta/inmunología , Peces Planos , Hipersensibilidad a los Alimentos/etiología , Immunoblotting , Inmunoglobulina E/inmunología , Inmunoglobulina E/metabolismo , Inmunoglobulina G/inmunología , Inmunoglobulina G/metabolismo , Lípidos/química , Lípidos/farmacocinética , Ratones Endogámicos BALB C , Parvalbúminas/inmunología , Parvalbúminas/metabolismo , Pepsina A/metabolismo
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