Effects of grape seed proanthocyanidin B2 pretreatment on oxidative stress and renal tubular epithelial cell apoptosis after renal ischemia reperfusion in mice
Acta cir. bras
; Acta cir. bras;35(8): e202000802, 2020. tab, graf
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| ID: biblio-1130668
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ABSTRACT
Abstract Purpose To investigate the effects of grape seed proanthocyanidin B2 (GSPB2) preconditioning on oxidative stress and apoptosis of renal tubular epithelial cells in mice after renal ischemia-reperfusion (RIR). Methods Forty male ICR mice were randomly divided into 4 groups Group A mice were treated with right nephrectomy. Group B right kidney was resected and the left renal vessel was clamped for 45 minutes. Group C mice were intraperitoneally injected with GSPB2 before RIR established. Group D mice were intraperitoneally injected with GSPB2 plus brusatol before RIR established. Creatinine and urea nitrogen of mice were determined. Pathological and morphological changes of kidney were checked. Expressions of Nrf-2, HO-1, cleaved-caspase3 were detected by Western-blot. Results Compared to Group B, morphology and pathological damages of renal tissue were less serious in Group C. Western-blot showed that expressions of Nrf-2 and HO-1 in Group C were obviously higher than those in Group B. The expression of cleaved-caspase3 in Group C was significantly lower than that in Group B. Conclusion GSPB2 preconditioning could attenuate renal oxidative stress injury and renal tubular epithelial cell apoptosis by up-regulating expressions of Nrf-2 and HO-1 and down-regulating the expression of cleaved-caspase-3, but the protective effect could be reversed by brusatol.
Palabras clave
Texto completo:
1
Banco de datos:
LILACS
Asunto principal:
Daño por Reperfusión
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Apoptosis
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Estrés Oxidativo
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Proantocianidinas
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Extracto de Semillas de Uva
Límite:
Animals
Idioma:
En
Revista:
Acta cir. bras
Asunto de la revista:
Cirurgia Geral
/
Procedimentos Cir£rgicos Operat¢rios
Año:
2020
Tipo del documento:
Article
País de afiliación:
China