Sperm/DNA interaction: integration of foreign DNA sequences in the mouse sperm genome.
J Reprod Immunol
; 41(1-2): 187-96, 1998 Dec.
Article
en En
| MEDLINE
| ID: mdl-10213310
Foreign DNA is spontaneously taken up by mouse epididymal sperm cells and is further internalized into nuclei. The interaction and/or internalization of the exogenous DNA triggers the activation of sperm endogenous nucleases which mediate rearrangements of the internalized DNA. Foreign DNA sequences are found to be tightly bound to the sperm nuclear scaffold, and to undergo a recombination process with the sperm chromosomal DNA. Sequence analysis of randomly selected clones from a library of sperm genomic DNA transformed with pSV2CAT plasmid showed that foreign sequences were integrated in a unique site of the sperm genome. Preliminary results suggest that the integration process is mediated by a retrotranscription step.
Buscar en Google
Banco de datos:
MEDLINE
Asunto principal:
Espermatozoides
/
Transformación Genética
Límite:
Animals
/
Humans
/
Male
Idioma:
En
Revista:
J Reprod Immunol
Año:
1998
Tipo del documento:
Article
País de afiliación:
Italia