Rapid serologic diagnosis of dengue virus infection using a commercial capture ELISA that distinguishes primary and secondary infections.
Am J Trop Med Hyg
; 60(4): 693-8, 1999 Apr.
Article
en En
| MEDLINE
| ID: mdl-10348250
ABSTRACT
A commercial capture ELISA for specific IgM and IgG antibodies produced during dengue infection (PanBio Dengue Duo) showed excellent sensitivity (99%, n = 78) using sera collected at hospital discharge compared with established ELISA and hemagglutination inhibition (HAI) assays. Furthermore, the ELISA was able to diagnose 79% of the dengue cases using sera collected at hospital admission. The ELISA also showed high specificity (92%) in paired sera from patients without flavivirus infection (n = 26), although 45% of the patients with Japanese encephalitis (n = 20) showed elevation of IgG but not IgM. The IgG capture ELISA showed good correlation with the HAI assay (r = 0.83, P < 0.0001), and IgG levels could be used to distinguish between primary and secondary infection, with 100% of primary infections and 96% of secondary infections being correctly classified. This ELISA should prove useful in the clinical diagnosis of dengue infections.
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Banco de datos:
MEDLINE
Asunto principal:
Ensayo de Inmunoadsorción Enzimática
/
Dengue
/
Virus del Dengue
/
Anticuerpos Antivirales
Tipo de estudio:
Diagnostic_studies
/
Evaluation_studies
Límite:
Child
/
Humans
Idioma:
En
Revista:
Am J Trop Med Hyg
Año:
1999
Tipo del documento:
Article
País de afiliación:
Tailandia