Reduction in water and metabolite apparent diffusion coefficients during energy failure involves cation-dependent mechanisms. A proton nuclear magnetic resonance study of rat cortical brain slices.
J Cereb Blood Flow Metab
; 20(2): 405-11, 2000 Feb.
Article
en En
| MEDLINE
| ID: mdl-10698079
ABSTRACT
Proton (1H) nuclear magnetic resonance (NMR) diffusion spectroscopy was used to assess apparent diffusion coefficients (ADCs) in rat brain slices. Aglycemic hypoxia caused reductions in the ADC of N-acetylaspartate (NAA) (0.15 to 0.09 x 10(-3) mm2/s) and "slow" diffusion coefficient (D2) of tissue water (0.51 to 0.37 x 10(-3) mm2/s), together with a 32+/-11% increase in tissue water volume, attributable to tissue swelling. The ADC and D2 reductions were diminished, however, by removing external Ca2+, and under 10 mmol/L Mg2+, normoxic diffusion coefficients persisted until 40 minutes of hypoxia. The data suggest that the shift of water into the intracellular space alone cannot satisfactorily explain the reduced cerebral diffusion upon energy failure and that external Mg2+ and Ca2+ play crucial modulatory roles.
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Banco de datos:
MEDLINE
Asunto principal:
Agua
/
Corteza Cerebral
/
Metabolismo Energético
Límite:
Animals
Idioma:
En
Revista:
J Cereb Blood Flow Metab
Año:
2000
Tipo del documento:
Article
País de afiliación:
Finlandia