Loss of interferon alpha and interferon tau-induced antiviral protection in interferon regulatory factor-2 DNA-binding domain dominant negative mutants.
Antiviral Res
; 46(3): 207-13, 2000 Jun.
Article
en En
| MEDLINE
| ID: mdl-10867158
ABSTRACT
The role of the interferon regulatory factory (IRF) family of transcription factors in regulation of interferon alpha and interferon tau antiviral activity was investigated using a dominant negative mutant of IRF-2. The IRF-2 DNA binding domain (DBD), without the C-terminal regulatory region, was stably transfected into myeloid U937 cells. Expression of the IRF-2 DBD resulted in an increase in constitutive 2'5' oligoadenylate synthetase (OAS) levels, indicative of an active repressive mechanism, but was not sufficient to protect cells from challenge with vesicular stomatitis virus. Treatment of the DBD clones with interferons alpha A and tau failed to upregulate 2'5' OAS expression and did not elicit an antiviral response. While interferon alpha A was more sensitive than interferon tau to the inhibitory effects of the IRF-2 DBD, IRF-mediated gene induction is involved in successful interferon alpha and tau-induced anti-VSV activity.
Buscar en Google
Banco de datos:
MEDLINE
Asunto principal:
Antivirales
/
Proteínas Gestacionales
/
Proteínas Represoras
/
Factores de Transcripción
/
Interferón Tipo I
/
Interferón-alfa
/
Virus de la Estomatitis Vesicular Indiana
/
Proteínas de Unión al ADN
Límite:
Humans
Idioma:
En
Revista:
Antiviral Res
Año:
2000
Tipo del documento:
Article
País de afiliación:
Estados Unidos