Survival of free DNA encoding antibiotic resistance from transgenic maize and the transformation activity of DNA in ovine saliva, ovine rumen fluid and silage effluent.
FEMS Microbiol Lett
; 191(1): 71-7, 2000 Oct 01.
Article
en En
| MEDLINE
| ID: mdl-11004402
ABSTRACT
To assess the likelihood that the bla gene present in a transgenic maize line may transfer from plant material to the microflora associated with animal feeds, we have examined the survival of free DNA in maize silage effluent, ovine rumen fluid and ovine saliva. Plasmid DNA that had previously been exposed to freshly sampled ovine saliva was capable of transforming competent Escherichia coli cells to ampicillin resistance even after 24 h, implying that DNA released from the diet could provide a source of transforming DNA in the oral cavity of sheep. Although target DNA sequences could be amplified by polymerase chain reaction from plasmid DNA after a 30-min incubation in silage effluent and rumen contents, only short term biological activity, lasting less than 1 min, was observed in these environments, as shown by transformation to antibiotic resistance. These experiments were performed under in vitro conditions; therefore further studies are needed to elucidate the biological significance of free DNA in the rumen and oral cavities of sheep and in silage effluent.
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Banco de datos:
MEDLINE
Asunto principal:
Transformación Bacteriana
/
ADN
/
Farmacorresistencia Microbiana
/
Plantas Modificadas Genéticamente
/
Zea mays
Límite:
Animals
Idioma:
En
Revista:
FEMS Microbiol Lett
Año:
2000
Tipo del documento:
Article
País de afiliación:
Reino Unido