Influence of decorin expression on transforming growth factor-beta-mediated collagen gel retraction and biglycan induction.
Matrix Biol
; 19(7): 631-6, 2000 Dec.
Article
en En
| MEDLINE
| ID: mdl-11102752
ABSTRACT
Complex formation of transforming growth factor-beta (TGF-beta) with the small proteoglycan decorin has been considered to inactivate the cytokine. However, neither the TGF-beta-mediated stimulation of the retraction of collagen lattices in culture nor the enhanced transcription of biglycan were influenced by an excess of native decorin in the culture medium. In contrast, when MG-63 osteosarcoma cells were transfected with sense- or antisense-decorin-cDNA, which led to an over- or under-expression of the proteoglycan, they responded to TGF-beta differently. An inverse correlation between decorin expression and the TGF-beta-mediated stimulation of collagen gel retraction and biglycan induction, respectively, was found. These results are best explained by assuming that decorin is not inactivating but sequestering TGF-beta in the extracellular matrix.
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Banco de datos:
MEDLINE
Asunto principal:
Proteoglicanos
/
Factor de Crecimiento Transformador beta
/
Colágeno
Límite:
Humans
Idioma:
En
Revista:
Matrix Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
/
BIOQUIMICA
Año:
2000
Tipo del documento:
Article
País de afiliación:
Alemania