A role for FAK in the Concanavalin A-dependent secretion of matrix metalloproteinase-2 and -9.
Oncogene
; 19(48): 5539-42, 2000 Nov 16.
Article
en En
| MEDLINE
| ID: mdl-11114732
ABSTRACT
To study the signaling pathway critical for the secretion of matrix metalloproteinases (MMPs), we examined the role of focal adhesion kinase (FAK) in Concanavalin A (Con A)-stimulated cells. We established a cell line in which FAK gene was conditionally inducible by use of FAK-null fibroblasts and the tetracycline repression system. In this cell line, FAK expression was undetectable in the presence of tetracycline but induced within 1 day by the removal of the drug. We found that FAK expression augmented the Con A-dependent secretion of MMP-9 and MMP-2. In contrast, proteolytic activation of MMP-2 by Con A-treatment did not require FAK expression. In addition, activation of MMP-secretion and tyrosine phosphorylation of FAK by Con A, but not the proteolytic activation of MMP-2, required attachment of the cells to the extracellular matrix. Taken together, our results suggest that the FAK signaling pathway play a pivotal role in the secretion of MMPs.
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Banco de datos:
MEDLINE
Asunto principal:
Proteínas Tirosina Quinasas
/
Concanavalina A
/
Metaloproteinasa 2 de la Matriz
/
Metaloproteinasa 9 de la Matriz
Límite:
Animals
Idioma:
En
Revista:
Oncogene
Asunto de la revista:
BIOLOGIA MOLECULAR
/
NEOPLASIAS
Año:
2000
Tipo del documento:
Article
País de afiliación:
Japón