Highly specific antiangiogenic therapy is effective in suppressing growth of experimental Wilms tumors.
J Pediatr Surg
; 36(2): 357-61, 2001 Feb.
Article
en En
| MEDLINE
| ID: mdl-11172434
ABSTRACT
BACKGROUND/PURPOSE:
Pathologic angiogenesis in tumors is a potential target for novel therapies. Vascular endothelial growth factor (VEGF) is an angiogenic promoter present in a wide variety of human tumors. VEGF is expressed as 4 isoforms; one of these, VEGF165, predominates in human tumors. The authors hypothesized that antagonism of VEGF165 by a specific aptamer would block tumor growth in an experimental model of Wilms tumor.METHODS:
VEGF isoform expression in clinical (n = 2) and experimental tumors were evaluated by reverse transcription polymerase chain reaction (RT-PCR). Tumors were induced in NCR nude mice (n = 32) by intrarenal injection of 10(6) cultured Wilms tumor cells. At 1 week, aptamer (n = 16) or vehicle (n = 16) treatment was started and continued daily for 5 weeks.RESULTS:
At 6 weeks tumors weighed 84% less in treated versus control animals (0.69 v 4.41 g; P <.028), without observed adverse effects and similar to suppression previously reported using nonisoform-specific anti-VEGF antibody (94% to 96%).CONCLUSIONS:
Anti-VEGF165 aptamer effectively suppressed primary tumor growth in experimental animals with no observed adverse effects. Development of highly specific antiangiogenic therapies may be of particular benefit to pediatric patients.
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Banco de datos:
MEDLINE
Asunto principal:
Tumor de Wilms
/
Inhibidores de la Angiogénesis
/
Neoplasias Renales
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
J Pediatr Surg
Año:
2001
Tipo del documento:
Article
País de afiliación:
Estados Unidos