[Use of colloid gold conjugates for identification of actins of various origin]. / Ispol'zovanie kon''ugatov kolloidnogo zolota dlia identifikatsii aktinov razlichnogo proiskhozhdeniia.

ABSTRACT

Some optimized methods of purifying actin from animal, plant, and bacterial cells were developed. Variants of the preparation of antiactin antibodies are described which use both traditional methods of immunization and phase display technology and antigen adsorption on colloidal gold particles. The conjugates of colloidal gold with phalloidin and heavy meromyosin as well as with antibodies were proposed. It was shown that these markers make it possible to reliably identify actins of different origin by the methods of light, and electron microscopy and dot-analysis.