[Use of colloid gold conjugates for identification of actins of various origin]. / Ispol'zovanie kon''ugatov kolloidnogo zolota dlia identifikatsii aktinov razlichnogo proiskhozhdeniia.
Biofizika
; 47(4): 632-40, 2002.
Article
en Ru
| MEDLINE
| ID: mdl-12298199
ABSTRACT
Some optimized methods of purifying actin from animal, plant, and bacterial cells were developed. Variants of the preparation of antiactin antibodies are described which use both traditional methods of immunization and phase display technology and antigen adsorption on colloidal gold particles. The conjugates of colloidal gold with phalloidin and heavy meromyosin as well as with antibodies were proposed. It was shown that these markers make it possible to reliably identify actins of different origin by the methods of light, and electron microscopy and dot-analysis.
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Banco de datos:
MEDLINE
Asunto principal:
Actinas
/
Oro Coloide
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
Idioma:
Ru
Revista:
Biofizika
Año:
2002
Tipo del documento:
Article