Human eosinophils express and release IL-13 following CD28-dependent activation.
J Leukoc Biol
; 72(4): 769-79, 2002 Oct.
Article
en En
| MEDLINE
| ID: mdl-12377947
ABSTRACT
Human eosinophils produce a large number of cytokines, including immunoregulatory cytokines. Given that eosinophils store and release interleukin (IL)-4, a key cytokine in the pathogenesis of allergic inflammation, and that IL-4 and IL-13 share common biological functions, we investigated the possibility that IL-13 may be synthesized by these cells. Using flow cytometry and immunocytochemistry, we show that eosinophils synthesize and store IL-13. Granule localization was demonstrated after subcellular fractionation, and IL-13 immunoreactivity was localized to crystalloid, granule-enriched fractions. Furthermore, electron microscopic analyses specifically localized IL-13 to the dense cores of bicompartmental secondary granules. Upon CD28 ligation, IL-13 was released by eosinophils, whereas a combination of CD28 and immunoglobulin A complexes resulted in decreased IL-13 secretion. Furthermore, eosinophil-derived IL-13 exerts a biological effect, inducing CD23 expression on B cells. By having the capacity to synthesize and release IL-13, eosinophils may participate in the development and maintenance of the T helper cell type 2 response, a prominent feature of allergic diseases.
Buscar en Google
Banco de datos:
MEDLINE
Asunto principal:
Síndrome Hipereosinofílico
/
Antígenos CD28
/
Interleucina-13
/
Eosinófilos
Límite:
Humans
Idioma:
En
Revista:
J Leukoc Biol
Año:
2002
Tipo del documento:
Article
País de afiliación:
Francia