Immunocytological localization of two enzymes involved in berberine biosynthesis.
Planta
; 216(1): 57-63, 2002 Nov.
Article
en En
| MEDLINE
| ID: mdl-12430014
ABSTRACT
Using post-embedding immunogold techniques the cytological localization of the two branchpoint enzymes of isoquinoline biosynthesis, berberine bridge enzyme (BBE) and (S)-tetrahydroprotoberberine oxidase (STOX), was demonstrated. Electron-microscopic examination revealed their exclusive compartmentation within vesicles. After these vesicles have fused with the central vacuole, they release their contents, resulting in a characteristic electron-dense precipitate at the tonoplast. Vesicles of similar structure could be identified in young meristematic tissues of roots or shoots from different Berberis species and Papaver somniferum L. The appearance of electron-dense osmiophilic material is strictly correlated with the alkaloid content of the tissue. Immunocytological staining of P. somniferum tissue with antibodies directed against BBE led to a characteristic labeling of electron-dense aggregates in idioblasts that are not connected to the laticifer system. This localization demonstrates the strictly cytological separation of benzophenanthridine and morphine biosyntheses within this plant.
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Banco de datos:
MEDLINE
Asunto principal:
Oxidorreductasas
/
Oxidorreductasas N-Desmetilantes
/
Plantas
/
Berberina
/
Bencilisoquinolinas
/
Oxidorreductasas actuantes sobre Donantes de Grupo CH-CH
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Planta
Año:
2002
Tipo del documento:
Article
País de afiliación:
Alemania