Crystallization and preliminary structure determination of the C-terminal truncated domain of the S-layer protein SbsC.
Acta Crystallogr D Biol Crystallogr
; 59(Pt 8): 1466-8, 2003 Aug.
Article
en En
| MEDLINE
| ID: mdl-12876353
ABSTRACT
The C-terminal truncated form of the S-layer protein SbsC from Geobacillus stearothermophilus, rSbsC(31-844), has been crystallized by the vapour-diffusion method using polyethylene glycol 6000 as a precipitating agent. The crystals diffract to 3 A resolution using synchrotron radiation and belong to space group P2(1), with unit-cell parameters a = 57.24, b = 98.91, c = 108.62 A, beta = 94.34 degrees. One molecule is present in the asymmetric unit, which corresponds to a solvent content of 65%. Native and heavy-atom derivative data have been collected. The Pt derivative yielded two high-occupancy sites per molecule.
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Banco de datos:
MEDLINE
Asunto principal:
Geobacillus stearothermophilus
/
Proteínas Bacterianas
/
Glicoproteínas de Membrana
Idioma:
En
Revista:
Acta Crystallogr D Biol Crystallogr
Año:
2003
Tipo del documento:
Article
País de afiliación:
Austria