Codelivery of a DNA vaccine and a protein vaccine with aluminum phosphate stimulates a potent and multivalent immune response.
J Mol Med (Berl)
; 81(8): 502-10, 2003 Aug.
Article
en En
| MEDLINE
| ID: mdl-12879151
ABSTRACT
The study explores the possibility of efficiently codelivering DNA vaccines and protein-based vaccines by formulation with aluminum phosphate (AlPO4). When mixed with aluminum adjuvants, plasmid DNA bound tightly onto aluminum hydroxide [Al(OH)3] but not to AlPO4. Different doses of DNA vaccines formulated with AlPO4 [but not Al(OH)3] induced enhanced humoral responses and supported priming of MHC class I restricted cellular immunity. Different proteins mixed with the plasmid DNA vaccine in the AlPO4 formulation did not impair its immunogenicity. Coinjection of two different vaccine-relevant antigens in the same AlPO4 formulation, one as a DNA vaccine and the other as a recombinant protein, elicited polyvalent, humoral, and cellular immune responses to all antigens delivered. The isotype profiles of the induced humoral responses and the cytokine profiles of the specifically primed T cell responses indicated that the combined vaccines supported copriming of Th1- and Th2-biased as well as balanced responses. These findings indicate that the AlPO4 adjuvant, a widely accepted adjuvant in human vaccination practice, can be used to combine protein- and DNA-based vaccination to prime an enhanced and balanced specific immunity.
Buscar en Google
Banco de datos:
MEDLINE
Asunto principal:
Fosfatos
/
Adyuvantes Inmunológicos
/
Vacunas contra Hepatitis B
/
Linfocitos T Colaboradores-Inductores
/
Compuestos de Aluminio
/
Linfocitos T CD8-positivos
/
Vacunas de ADN
/
Anticuerpos contra la Hepatitis B
Límite:
Animals
/
Humans
Idioma:
En
Revista:
J Mol Med (Berl)
Asunto de la revista:
BIOLOGIA MOLECULAR
/
GENETICA MEDICA
Año:
2003
Tipo del documento:
Article
País de afiliación:
Alemania