[In vivo and vitro study of prostaglandins E2 and I2 participation in protective function of antacids on gastric mucosa]. / Badania in vivo i in vitro nad udzialem prostaglandyny E2 i I2 w ochronnym dzialaniu alkaliów na blone sluzowa zoladka.

ABSTRACT

Antacids apart from neutralization of hydrochloric acid possess the gastro-protective properties. In this paper, performed in vivo and in vitro, we studied the effects of antacids (aluminium and magnesium hydroxides) on secretion of PGE2 and 6-keto-PGF1 alpha (a stable metabolite of PGI2) by the gastric mucosa and isolated gastric mucosal cells of rats. We studied protective effects of these drugs on the gastric mucosa and isolated cells as well. It was shown that antacids stimulate generation of PGE2 and PGI2 in the gastric mucosa and protect it against alcohol-induced damage. Indomethacin given prior to antacids in a dose (1 mg/kg) which did not cause any gastric mucosal changes but inhibited generation of both prostaglandins, abolished protective effects of antacids. In comparison with control, surface epithelial cells of the gastric mucosa isolated from the stomachs of rats which were treated with antacids earlier, secreted significantly more PGE2 and PGI2, and were less damaged after incubation with 8% alcohol. Indomethacin inhibited secretion of prostaglandins by isolated surface epithelial cells of the gastric mucosa and abolished protective effects of antacids on these cells. The study confirms both in vivo and in vitro the protective properties of antacids and shows that prostaglandins participate in their action.