Induction of a pharmacologically active clonotypic B cell response directed to an immunogenic region of the human beta 2-adrenergic receptor.
Clin Exp Immunol
; 89(3): 461-7, 1992 Sep.
Article
en En
| MEDLINE
| ID: mdl-1381303
ABSTRACT
It has been reported that autoantibodies against the beta 2-adrenergic receptors are involved in the pathology of allergic disorders and of Chagas' disease. Therefore, the immune response against a peptide (H26Q) corresponding to the putative second extracellular loop of the human beta 2-adrenergic receptor, which could be a target for autoantibody attack, was analysed in view of its possible immunogenicity. The free peptide induced a T cell-mediated humoral response in the context of three different murine MHC haplotypes. The T cell epitope was found to be localized in the N-terminal region of the peptide. Highly specific T helper cells were capable of stimulating B cells with the potential to generate a large antibody repertoire reactive with the loop peptide. MoAbs were screened to analyse this B cell response for antibodies potentially interfering with receptor function and a MoAb was found that impaired ligand binding to the receptor.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Linfocitos B
/
Receptores Adrenérgicos beta
Límite:
Animals
Idioma:
En
Revista:
Clin Exp Immunol
Año:
1992
Tipo del documento:
Article
País de afiliación:
Francia