Validation of a cell-based screen for insulin receptor modulators by quantification of insulin receptor phosphorylation.
J Biomol Screen
; 8(4): 439-46, 2003 Aug.
Article
en En
| MEDLINE
| ID: mdl-14567796
ABSTRACT
Stimulation of a cell with insulin initiates a signal transduction cascade that results in cellular activities that include phosphorylation of the receptor itself. Measurement of the degree of phosphorylation can serve as a marker for receptor activation. Receptor phosphorylation has been measured using Western blot analysis, which is very low throughput and not easily quantifiable. The goal of this project was to develop a cell-based assay to measure receptor phosphorylation in high throughput. This report describes a cell-based assay for insulin receptor phosphorylation that is robust and amenable to high-volume screening in a microwell format.
Buscar en Google
Banco de datos:
MEDLINE
Asunto principal:
Receptor de Insulina
/
Transferencia Resonante de Energía de Fluorescencia
/
Insulina
Límite:
Animals
Idioma:
En
Revista:
J Biomol Screen
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2003
Tipo del documento:
Article
País de afiliación:
Estados Unidos