[Immunopotency of the recombinant urease B subunit vaccine of Helicobacter pylori after intranasal administration to mice].
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
; 19(1): 68-70, 2003 Jan.
Article
en Zh
| MEDLINE
| ID: mdl-15132912
ABSTRACT
AIM:
To observe the immunopotency of the recombinant urease B subunit (rUreB) of Helicobacter pylori after intranasal administration to mice. MDTHODS BALB/c mice were immunized intranasally with rUreB of 20 microg,10 microg and rUreB plus different adjuvants, such as cholera toxin B subunit (CTB), Escherichia coli heat labile enterotoxin B subunit (LTB) and carbopol respectively, four times at an intervals of 7 days. The serum and washing solution from gastric, intestinal, nasal and tracheal mucosas were collected in 7 days after final immunization. IgG and IgA antibodies specific for rUreB were detected by ELISA.RESULTS:
The levels of IgA and IgG antibodies in sera every groups of mice immunized intranasally were significantly increased compared with control group (P<0.01). Only the levels of serum IgG of mice immunized with 20 microg dose were higher than those of mice immunized with 10 microg dose. Carbopol could enhance the level of IgA antibodies in washing solution from mouse gastric mucosa after intranasal immunization. The efficacy of LTB as a nasal mucosal immuno-adjuvant was stronger than that of CTB.CONCLUSION:
CTB, LTB and carbopol can play the role of adjuvant in nasal mucosal immunization. Intranasal immunization with rUreB can induce not only serum IgG antibody production but also antibody responses of different mucosa. Thus intranasal inoculation is a convenient, effective and cheap immunization way.
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Banco de datos:
MEDLINE
Asunto principal:
Ureasa
/
Inmunoglobulina A
/
Inmunoglobulina G
/
Vacunas Bacterianas
/
Helicobacter pylori
Límite:
Animals
Idioma:
Zh
Revista:
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
Asunto de la revista:
ALERGIA E IMUNOLOGIA
Año:
2003
Tipo del documento:
Article
País de afiliación:
China