Role of H19 3' sequences in controlling H19 and Igf2 imprinting and expression.
Genomics
; 84(1): 59-68, 2004 Jul.
Article
en En
| MEDLINE
| ID: mdl-15203204
ABSTRACT
The regulation of H19 and Igf2 imprinting and expression depends on common elements. Using comparative analysis between human and mouse, we identified conserved regions 3' of the H19 transcription unit, including the H19/Igf2 endodermal enhancers and elements within a 4.2-kb domain between the H19 transcription unit and the enhancers. Transgene experiments implicate these elements in imprinting regulation. To establish whether they are required at the endogenous locus, first we replaced the endodermal enhancers with the alpha-fetoprotein endodermal enhancers (H19Afp). Second, we deleted the 4.2-kb region (H19delta4.2). Our analysis revealed that H19 and Igf2 imprinting and tissue-specific expression were maintained for both mutations, except for a slight reduction in paternal Igf2 expression from the H19Afp allele in liver. These results demonstrate that the H19 insulator can interact with heterologous enhancers to imprint Igf2. Furthermore, for H19, chromatin context or additional sequences possibly compensate for loss of conserved 3' elements.
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Banco de datos:
MEDLINE
Asunto principal:
Transcripción Genética
/
Factor II del Crecimiento Similar a la Insulina
/
Elementos de Facilitación Genéticos
/
Impresión Genómica
/
ARN no Traducido
/
Región de Flanqueo 3'
Tipo de estudio:
Prognostic_studies
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Genomics
Asunto de la revista:
GENETICA
Año:
2004
Tipo del documento:
Article
País de afiliación:
Estados Unidos