Gene probes for the detection of 6-deoxyhexose metabolism in secondary metabolite-producing streptomycetes.
FEMS Microbiol Lett
; 69(2): 185-9, 1992 Jan 01.
Article
en En
| MEDLINE
| ID: mdl-1537548
ABSTRACT
DNA probes were designed from the streptomycin production genes strDELM of Streptomyces griseus involved in the biosynthesis of the 6-deoxyhexose (6DOH) dihydrostreptose which could detect the genomic fragments coding for 6DOH formation in other actinomycetes strains. In about 70% of the 43 strains tested at least one signal could be detected with strD-, strE- or strLM-specific probes. Evidence is presented that the hybridizing genes are mostly clustered and probably engaged in the formation of secondary metabolites. Because of the wide-spread use of 6DOH constituents in natural products these probes should allow to detect a vast array of different secondary metabolic gene clusters in actinomycetes.
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Banco de datos:
MEDLINE
Asunto principal:
Streptomyces griseus
/
Desoxiazúcares
/
Sondas de ADN
/
Hexosas
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
FEMS Microbiol Lett
Año:
1992
Tipo del documento:
Article