The structure of a resuscitation-promoting factor domain from Mycobacterium tuberculosis shows homology to lysozymes.
Nat Struct Mol Biol
; 12(3): 270-3, 2005 Mar.
Article
en En
| MEDLINE
| ID: mdl-15723078
ABSTRACT
Resuscitation-promoting factor (RPF) proteins reactivate stationary-phase cultures of (G+C)-rich Gram-positive bacteria including the causative agent of tuberculosis, Mycobacterium tuberculosis. We report the solution structure of the RPF domain from M. tuberculosis Rv1009 (RpfB) solved by heteronuclear multidimensional NMR. Structural homology with various glycoside hydrolases suggested that RpfB cleaved oligosaccharides. Biochemical studies indicate that a conserved active site glutamate is important for resuscitation activity. These data, as well as the presence of a clear binding pocket for a large molecule, indicate that oligosaccharide cleavage is probably the signal for revival from dormancy.
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Banco de datos:
MEDLINE
Asunto principal:
Proteínas Bacterianas
/
Citocinas
/
Glicósido Hidrolasas
/
Mycobacterium tuberculosis
Idioma:
En
Revista:
Nat Struct Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2005
Tipo del documento:
Article
País de afiliación:
Reino Unido