Prostaglandin E(2) modulation of gene expression in an Atlantic salmon (Salmo salar) macrophage-like cell line (SHK-1).
Dev Comp Immunol
; 29(11): 951-63, 2005.
Article
en En
| MEDLINE
| ID: mdl-15936074
ABSTRACT
Following lipopolysaccharide (LPS)-stimulation of Atlantic salmon (Salmo salar) macrophage-like SHK-1 cells, prostaglandin E(2) (PGE(2)) exhibited dose-dependent inhibition of the antigen presenting molecules major histocompatability class I and II and the pro-inflammatory cytokine interleukin-1 beta gene expression. Prostaglandin E(2) was found to be stimulatory towards cyclooxygenase-2 (COX-2) expression at higher concentrations (1 x 10(-6) and 1 x 10(-8)M) and inhibitory at lower concentrations (1 x 10(-10) and 1 x 10(-12)M) after 4h exposure. After 24h exposure, however, LPS-induced COX-2 expression decreased and was completely inhibited by all PGE(2) concentrations (1 x 10(-6)-1 x 10(-10)M). Incubation of SHK-1 cells with LPS alone had no effect on tumour necrosis factor alpha (TNFalpha)-like gene or transforming growth factor beta-like gene expression after 4h, however, LPS and PGE(2) showed a synergistic effect on TNFalpha-like gene expression after 24h. This study provides evidence for the existence of a PGE(2)-mediated negative feedback mechanism in the control of PGs through down-regulation of COX-2, as well as for inflammatory responses by the down-regulation of both COX-2 and IL-1 beta. The differential regulation of immune-related genes under these conditions further demonstrates the usefulness of the SHK-1 cell line for studying aspects of salmonid immunology.
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Banco de datos:
MEDLINE
Asunto principal:
Dinoprostona
/
Salmo salar
Límite:
Animals
Idioma:
En
Revista:
Dev Comp Immunol
Año:
2005
Tipo del documento:
Article