Interaction of HIV-1 Gag with the clathrin-associated adaptor AP-2.
Virology
; 342(2): 190-200, 2005 Nov 25.
Article
en En
| MEDLINE
| ID: mdl-16139856
ABSTRACT
The envelope glycoprotein (Env) of HIV-1 interacts with the clathrin-associated adaptor complex AP-2 during the late phase of the viral replication cycle. Upon its synthesis, Env, therefore, is retrieved from the cellular surface unless internalization is inhibited by viral Gag. Here we demonstrate that not only Env, but also HIV-1 Gag, specifically binds to AP-2. Gag-AP-2 association was found to depend on tyrosine residue 132 and valine residue 135 at the matrix-capsid junction in the Gag polyprotein. Results of a morphological analysis of viral egress from cells expressing dominant-negative AP-2 suggest an involvement of AP-2 in confining HIV-1 exit to distinct microdomains. Further, particle release from AP-2-mutant cells was enhanced compared to release from wild-type cells but the infectivity of virus released from these cells was moderately reduced. Together these data attribute a role to the AP-2 complex in the regulation of HIV-1 assembly/release.
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Banco de datos:
MEDLINE
Asunto principal:
Productos del Gen gag
/
VIH-1
/
Complejo 2 de Proteína Adaptadora
Tipo de estudio:
Risk_factors_studies
Límite:
Humans
Idioma:
En
Revista:
Virology
Año:
2005
Tipo del documento:
Article
País de afiliación:
Estados Unidos