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Evaluation of different plasmid DNA delivery systems for immunization against HER2/neu in a transgenic murine model of mammary carcinoma.
Smorlesi, Arianna; Papalini, Francesca; Amici, Augusto; Orlando, Fiorenza; Pierpaoli, Sara; Mancini, Chiara; Provinciali, Mauro.
Afiliación
  • Smorlesi A; Laboratory of Tumor Immunology, Immunology Center, INRCA Gerontology Research Department, Via Birarelli 8, 60121 Ancona, Italy.
Vaccine ; 24(11): 1766-75, 2006 Mar 10.
Article en En | MEDLINE | ID: mdl-16288939
Studies of DNA vaccination against HER2/neu showed the effectiveness of immunization protocols in models of transplantable or spontaneous tumors; scarce information, however, has been provided to identify the procedure of DNA administration that more effectively contributes to the activation of immune system against spontaneously arising HER2/neu-positive tumors. We compared the effectiveness of different procedures of DNA vaccine delivery (intradermic injection (ID), gene gun (GG) delivery and intramuscular injection (IM) alone or with electroporation) in a murine transgenic model of mammary carcinoma overexpressing HER2/neu. We highlighted the role of DNA delivery system in the success of DNA vaccination showing that, among the analysed methods, intramuscular injection of the vaccine, particularly when associated to electroporation, elicits a better protection against HER2/neu spontaneous tumor development inducing antibody and cell-mediated immune responsiveness against HER2/neu and a Th1 polarization of the immune response.
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Banco de datos: MEDLINE Asunto principal: Plásmidos / Genes erbB-2 / Vacunas contra el Cáncer / Vacunas de ADN / Neoplasias Mamarias Experimentales Límite: Animals Idioma: En Revista: Vaccine Año: 2006 Tipo del documento: Article País de afiliación: Italia
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Banco de datos: MEDLINE Asunto principal: Plásmidos / Genes erbB-2 / Vacunas contra el Cáncer / Vacunas de ADN / Neoplasias Mamarias Experimentales Límite: Animals Idioma: En Revista: Vaccine Año: 2006 Tipo del documento: Article País de afiliación: Italia