Influence of the duration of ischemia and reperfusion on infarct volume and microvascular damage in mice.
Neurol Res
; 28(2): 200-5, 2006 Mar.
Article
en En
| MEDLINE
| ID: mdl-16551441
ABSTRACT
OBJECTIVES:
Focal cerebral ischemia is responsible for alterations of vascular permeability, and the loss of microvascular integrity is a primary source of subsequent hemorrhages. We evaluated the influence of different durations of ischemia and reperfusion on infarction size and microvascular damage after focal cerebral ischemia in the mouse.METHODS:
C57BL/6 mice (n=39) were subjected to focal cerebral ischemia (I) and reperfusion (R). Consecutive brain sections were analysed for infarction volumes (Nissl-staining) and for collagen type IV (immunohistochemistry and western blot).RESULTS:
Infarction size (percentage of the infarction volume versus ipsilateral hemisphere) increased with total time of ischemia and reperfusion 19+/-2% (I3R0), 30+/-2% (I3R3), 36+/-4% (I3R12), 41+/-4% (I1R24), 45+/-6% (I2R24) and 58+/-2% (I3R24). The ischemic hemispheres showed a significant progressive reduction of collagen type IV positive vessels (ischemic versus non-ischemic contralateral area) 90+/-3% (I3R0), 88+/-1% (I3R3), 82+/-3% (I3R12), 85+/-3% (I1R24), 79+/-3% (I2R24), 72+/-2% (I3R24).CONCLUSIONS:
Both prolonged ischemia and reperfusion lead to an increased infarction volume, as well as progressive microvascular damage.
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Banco de datos:
MEDLINE
Asunto principal:
Daño por Reperfusión
/
Isquemia Encefálica
/
Arteria Cerebral Media
/
Infarto Encefálico
/
Infarto de la Arteria Cerebral Media
/
Microcirculación
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Neurol Res
Año:
2006
Tipo del documento:
Article
País de afiliación:
Alemania