Distribution of CESP-1 protein in the corneal endothelium and other tissues.
Invest Ophthalmol Vis Sci
; 47(4): 1397-403, 2006 Apr.
Article
en En
| MEDLINE
| ID: mdl-16565373
ABSTRACT
PURPOSE:
The gene expression profile of human corneal endothelium (CE) was established with the gene signature system. A novel gene, GS3582, was abundantly transcribed in the CE compared with other tissues according to a human gene expression database. This protein was designated corneal endothelium-specific protein (CESP)-1. The tissue distribution and subcellular localization of CESP-1 was assessed in humans and mice, to investigate its physiological function.METHODS:
Rabbit and mouse CESP-1 cDNAs were cloned, and a polyclonal anti-human CESP-1 antibody (Ab) and anti-mouse N- or C-terminal ovary-specific acidic protein (OSAP)-1 Ab were produced. CESP-1 expression was investigated in human and mouse corneas by Western blot and/or immunohistochemical analysis. The distribution of CESP-1 in human tissues was also examined by Western blot analysis. To identify the subcellular localization of CESP-1, cultured human CE was colabeled with anti-human CESP-1 Ab and anti-cytochrome c monoclonal Ab or anti-GRP78 monoclonal Ab for confocal microscopy.RESULTS:
The rabbit and mouse CESP-1 cDNA sequences contained an open reading frame coding 242 and 283 amino acids, respectively. Mouse CESP-1 was entirely consistent with mouse OSAP. Western blot analysis showed that CESP-1 was expressed in the human corneal epithelium, CE, cultured CE, brain, testis, and ovary. Mouse CESP-1 was also expressed in mouse corneal epithelium and CE with anti-mouse C- but not N-terminal OSAP Ab according to immunohistochemical analysis. Subcellular localization of CESP-1 to the mitochondria was demonstrated in cultured human CE. The N-terminal of CESP-1, possessing a mitochondrial targeting sequence, may be processed after the protein is imported into the mitochondria.CONCLUSIONS:
CESP-1 was distributed in the corneal epithelium, the CE and cultured human CE, as well as the brain, testis, and ovary. CESP-1 was localized in the mitochondria of cultured human CE. These findings may provide some clues about the physiological function of CESP-1.
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Banco de datos:
MEDLINE
Asunto principal:
Ovario
/
Testículo
/
Encéfalo
/
Endotelio Corneal
/
Proteínas del Ojo
Tipo de estudio:
Prognostic_studies
Límite:
Adult
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Aged
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Animals
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Female
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Humans
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Male
/
Middle aged
Idioma:
En
Revista:
Invest Ophthalmol Vis Sci
Año:
2006
Tipo del documento:
Article
País de afiliación:
Japón