Characterization of Streptococcus thermophilus host range phage mutants.
Appl Environ Microbiol
; 72(4): 3036-41, 2006 Apr.
Article
en En
| MEDLINE
| ID: mdl-16598014
ABSTRACT
To investigate phage-host interactions in Streptococcus thermophilus, a phage-resistant derivative (SMQ-301R) was obtained by challenging a Tn917 library of phage-sensitive strain S. thermophilus SMQ-301 with virulent phage DT1. Mutants of phages DT1 and MD2 capable of infecting SMQ-301 and SMQ-301R were isolated at a frequency of 10(-6). Four host range phage mutants were analyzed further and compared to the two wild-type phages. Altogether, three genes (orf15, orf17, and orf18) contained point mutations leading to amino acid substitutions and were responsible for the expanded host range. These three proteins were also identified in both phages by N-terminal sequencing and/or matrix-assisted laser desorption ionization-time-of-flight mass spectrometry. The results suggest that at least three phage structural proteins may be involved in phage-host interactions in S. thermophilus.
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1
Banco de datos:
MEDLINE
Asunto principal:
Proteínas Estructurales Virales
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Fagos de Streptococcus
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Streptococcus thermophilus
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Mutación
Idioma:
En
Revista:
Appl Environ Microbiol
Año:
2006
Tipo del documento:
Article