Development of monoclonal antibodies against pirimiphos-methyl and their application to IC-ELISA.
J Agric Food Chem
; 54(13): 4551-6, 2006 Jun 28.
Article
en En
| MEDLINE
| ID: mdl-16786997
ABSTRACT
To detect the organophosphorus (OP) pesticide pirimiphos-methyl in grain samples, a monoclonal antibody-based indirect competitive enzyme-linked immunosorbent assay (IC-ELISA) was developed and optimized. By the active esters method, pirimiphos-methyl hapten A was conjugated to keyhole limpet hemocyanin to be used as the immunogen for the production of monoclonal antibodies, and pirimiphos-methyl hapten B was conjugated to ovalbumin to be used as coating antigen. By using the monoclonal antibody and the coating antigen, an IC-ELISA has been developed. Under the established optimized conditions, the IC-ELISA showed an IC50 of 4.2 ng/mL with a detection limit of 0.07 ng/mL. The IC-ELISA showed negligible cross-reactivity with other OP pesticides except with pirimiphos-ethyl. Recoveries of pirimiphos-methyl from spiked grain samples ranged from 83 to 96%.
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Banco de datos:
MEDLINE
Asunto principal:
Compuestos Organotiofosforados
/
Ensayo de Inmunoadsorción Enzimática
/
Insecticidas
/
Anticuerpos Monoclonales
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
J Agric Food Chem
Año:
2006
Tipo del documento:
Article
País de afiliación:
Corea del Sur