Alpha-tocopherol induces calnexin in renal tubular cells: another protective mechanism against free radical-induced cellular damage.
Arch Biochem Biophys
; 453(2): 168-78, 2006 Sep 15.
Article
en En
| MEDLINE
| ID: mdl-16908007
ABSTRACT
Pre-administration of alpha-tocopherol is protective against oxidative renal tubular damage and subsequent carcinogenesis by ferric nitrilotriacetate (Fe-NTA) in rats. We searched for mechanisms other than the scavenging effect of alpha-tocopherol with microarray analyses, which implicated calnexin, a chaperone for glycoproteins. Renal mRNA levels of calnexin significantly increased 3h after an injection of Fe-NTA in rats fed a standard diet whereas those fed an alpha-tocopherol-supplemented diet showed an increase prior to injection, but after injection showed a decrease in renal calnexin mRNA levels, with unaltered protein levels. In experiments using LLC-PK1 cells, addition of alpha-tocopherol was protective against oxidative stress by H2O2, concomitant with calnexin induction. Knockdown of calnexin by siRNA significantly reduced this protection. Furthermore, COS-7 cells transfected with the calnexin gene were more resistant to H2O2. Together with the fact that alpha-tocopherol induced N-acetylglucosaminyltransferase 3, our data suggest that alpha-tocopherol modifies glycoprotein metabolism partially by conferring mild ER stress. This adds another molecular mechanism of alpha-tocopherol toward cancer prevention.
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Banco de datos:
MEDLINE
Asunto principal:
Lesiones Precancerosas
/
Estrés Oxidativo
/
Alfa-Tocoferol
/
Neoplasias Renales
/
Túbulos Renales
Límite:
Animals
Idioma:
En
Revista:
Arch Biochem Biophys
Año:
2006
Tipo del documento:
Article
País de afiliación:
Japón