Rad52 and Rad59 exhibit both overlapping and distinct functions.
DNA Repair (Amst)
; 6(1): 27-37, 2007 Jan 04.
Article
en En
| MEDLINE
| ID: mdl-16987715
ABSTRACT
Homologous recombination is an important pathway for the repair of DNA double-strand breaks (DSBs). In the yeast Saccharomyces cerevisiae, Rad52 is a central recombination protein, whereas its paralogue, Rad59, plays a more subtle role in homologous recombination. Both proteins can mediate annealing of complementary single-stranded DNA in vitro, but only Rad52 interacts with replication protein A and the Rad51 recombinase. We have studied the functional overlap between Rad52 and Rad59 in living cells using chimeras of the two proteins and site-directed mutagenesis. We find that Rad52 and Rad59 have both overlapping as well as separate functions in DSB repair. Importantly, the N-terminus of Rad52 possesses functions not supplied by Rad59, which may account for its central role in homologous recombination.
Buscar en Google
Banco de datos:
MEDLINE
Asunto principal:
Saccharomyces cerevisiae
/
ADN de Cadena Simple
/
Proteínas de Saccharomyces cerevisiae
/
Proteínas de Unión al ADN
/
Reparación del ADN
/
Proteína Recombinante y Reparadora de ADN Rad52
Idioma:
En
Revista:
DNA Repair (Amst)
Asunto de la revista:
BIOLOGIA MOLECULAR
/
BIOQUIMICA
Año:
2007
Tipo del documento:
Article
País de afiliación:
Estados Unidos