Plasmodium falciparum: a novel method for analyzing haplotypes in mixed infections.
Exp Parasitol
; 115(3): 233-41, 2007 Mar.
Article
en En
| MEDLINE
| ID: mdl-17049516
ABSTRACT
Studying the population genetics of Plasmodium falciparum is necessary for understanding the spread of drug resistance. However, these studies are hampered by the inability to determine haplotypes from patient samples that contain multiple parasite populations. Therefore, we have developed a method for separating for genetic analysis the individual strains in a mixed infection. We amplified a 6 kb region of chromosome 4, including the dihydrofolate reductase gene and upstream microsatellite markers. This PCR product was inserted by recombination into a gapped yeast shuttle plasmid containing both selectable and counter-selectable markers. Because each plasmid contains only one insert and each yeast colony contains only one plasmid, the individual strains are now separate. We analyzed mixtures of 3D7, K1, and Dd2 DNA and correctly identified a haplotype in each case.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Plasmodium falciparum
/
Haplotipos
/
Malaria Falciparum
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Exp Parasitol
Año:
2007
Tipo del documento:
Article
País de afiliación:
Estados Unidos