The detection application of CdS quantum dots in labeling DNA molecules.
Biomed Mater
; 1(2): 81-4, 2006 Jun.
Article
en En
| MEDLINE
| ID: mdl-18460760
ABSTRACT
CdS nanoparticles with narrow symmetric emission and stable photochemical properties are synthesized. The particles are labeled on the probe DNA molecules due to the electrostatic interaction between the amidocyanogen DNA molecules with positive charges and the free carboxyl on the surface of CdS nanocrystals with negative charges. We find that the fluorescence intensity of the CdS-DNA decreases with the increasing temperature. The target DNA molecules immobilized on the quartz slides in two patterns, random and linear array, are successfully detected by the CdS labeled probe DNA molecules using the fluorescence of the particles. This may offer a more stable and quantitative mode of fluorescence in-situ research and biologic analysis.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Espectrometría de Fluorescencia
/
Coloración y Etiquetado
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Sulfuros
/
Sondas de ADN
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Hibridación Fluorescente in Situ
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Compuestos de Cadmio
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Puntos Cuánticos
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Biomed Mater
Asunto de la revista:
ENGENHARIA BIOMEDICA
Año:
2006
Tipo del documento:
Article