The role of chronic infection in children with otitis media with effusion: evidence for intracellular persistence of bacteria.
Otolaryngol Head Neck Surg
; 138(6): 778-81, 2008 Jun.
Article
en En
| MEDLINE
| ID: mdl-18503854
ABSTRACT
OBJECTIVE:
Demonstrate mucosal bacterial infection in children with otitis media with effusion (OME). STUDY DESIGN ANDSETTING:
Middle ear mucosal biopsies from 11 children with OME were examined for bacteria utilizing transmission electron microscopy. This was correlated with standard culture and polymerase chain reaction (PCR) of middle ear effusions.RESULTS:
Gram-positive coccal bacteria were demonstrated in middle ear mucosal epithelial cells of 4 of 11 (36%) children. Morphological appearance of bacteria and detection of pneumolysin DNA by PCR in middle ear fluid suggests a role for persistent intracellular infection with Streptococcus pneumoniae and other gram-positive cocci in some cases of OME.CONCLUSION:
Intracellular bacterial infection of middle ear mucosal epithelial cells in children with OME may be an important mechanism for bacterial persistence, and contribute to inflammation and mucus production in the pathogenesis of this condition.SIGNIFICANCE:
Persistent intracellular infection is a novel paradigm for OME pathogenesis in children and may influence antibiotic effectiveness in treatment of this condition.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Otitis Media con Derrame
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Cocos Grampositivos
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Espacio Intracelular
Límite:
Child
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Child, preschool
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Female
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Humans
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Male
Idioma:
En
Revista:
Otolaryngol Head Neck Surg
Año:
2008
Tipo del documento:
Article