Evaluation of the use of UPLC-TOFMS with simultaneous [14C]-radioflow detection for drug metabolite profiling: application to propranolol metabolites in rat urine.
J Pharm Biomed Anal
; 48(1): 151-7, 2008 Sep 10.
Article
en En
| MEDLINE
| ID: mdl-18571352
ABSTRACT
The non-selective beta-adrenergic receptor antagonist propranolol [1-(isopropylamino)-3-(1-naphthoxy)-2-propanol] is metabolised extensively in vivo. Enumerating and identifying the many metabolites that result from multiple biotransformations provides a considerable analytical challenge, greatly aided by efficient chromatography coupled to sensitive mass spectrometric detection. Here the use of the newly introduced high-resolution technique of "ultra performance liquid chromatography" (UPLC) linked to quadrupole time-of-flight mass spectrometry (TOFMS) with simultaneous [(14)C]-radioflow detection was applied to rapid metabolite profiling. [14C]-propranolol, dosed intraperitoneally to rat at 25 mg kg(-1) and 200 microCi kg(-1) was used as a model compound for this evaluation. Some 14 metabolites were detected in the urine by this technique including a number of conjugated metabolites such as sulphates, several isobaric glucuronides and two novel di-glucuronides.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Propranolol
/
Espectrometría de Masas
/
Cromatografía Liquida
/
Antagonistas Adrenérgicos beta
Tipo de estudio:
Diagnostic_studies
/
Evaluation_studies
Límite:
Animals
Idioma:
En
Revista:
J Pharm Biomed Anal
Año:
2008
Tipo del documento:
Article
País de afiliación:
Reino Unido