Acetylation of Smc3 by Eco1 is required for S phase sister chromatid cohesion in both human and yeast.
Mol Cell
; 31(1): 143-51, 2008 Jul 11.
Article
en En
| MEDLINE
| ID: mdl-18614053
ABSTRACT
Sister chromatid cohesion is normally established in S phase in a process that depends on the cohesion establishment factor Eco1, a conserved acetyltransferase. However, due to the lack of known in vivo substrates, how Eco1 regulates cohesion is not understood. Here we report that yeast Eco1 and its human ortholog, ESCO1, both acetylate Smc3, a component of the cohesin complex that physically holds the sister chromatid together, at two conserved lysine residues. Mutating these lysine residues to a nonacetylatable form leads to increased loss of sister chromatid cohesion and genome instability in both yeast and human. In addition, we clarified that the acetyltransferase activity of Eco1 is essential for its function. Our study thus identified a molecular target for the acetyltransferase Eco1 and revealed that Smc3 acetylation is a conserved mechanism in regulating sister chromatid cohesion.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Proteoglicanos Tipo Condroitín Sulfato
/
Saccharomyces cerevisiae
/
Acetiltransferasas
/
Intercambio de Cromátides Hermanas
/
Proteínas Nucleares
/
Proteínas Cromosómicas no Histona
/
Fase S
/
Proteínas de Ciclo Celular
/
Proteínas de Saccharomyces cerevisiae
Tipo de estudio:
Prognostic_studies
Límite:
Humans
Idioma:
En
Revista:
Mol Cell
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2008
Tipo del documento:
Article
País de afiliación:
Estados Unidos