Rapid loss of dendritic cell and monocyte responses to TLR ligands following venipuncture.
J Immunol Methods
; 339(2): 132-40, 2008 Dec 31.
Article
en En
| MEDLINE
| ID: mdl-18848564
ABSTRACT
Blood samples from multiple sites are collected in multicenter trials, and frequently shipped to centralized laboratories for processing and comparable experimental evaluation. It is therefore of crucial interest to assess the preservation of immune cell functions after overnight shipment of whole blood. Here we evaluated the ability of pDCs, mDCs and monocytes to respond to TLR ligands at multiple timepoints following venipuncture as compared to immediate processing. Our results demonstrate a profound impairment of APC function, in particular of IFN-alpha production of pDCs, if whole blood was processed later than 6 h after venipuncture. Overnight shipment or extended rest of whole blood before processing therefore severely compromises the ability of APCs to respond to TLR ligands, and this has to be taken into consideration when designing multicenter trials.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Preservación Biológica
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Células Dendríticas
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Monocitos
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Flebotomía
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Receptores Toll-Like
Tipo de estudio:
Clinical_trials
Límite:
Humans
Idioma:
En
Revista:
J Immunol Methods
Año:
2008
Tipo del documento:
Article
País de afiliación:
Estados Unidos