Glutathione oxidase-like activity of glass beads, silica gel and anion-exchange resin modified with cobalt(III)-tetrakis(4-carboxyphenyl)porphine.
Talanta
; 46(6): 1583-90, 1998 Aug.
Article
en En
| MEDLINE
| ID: mdl-18967291
ABSTRACT
Silica gel and glass beads were modified by using acid chloride of metal-tetrakis(4-carboxyphenyl)porphine (M-TCPP) through a peptide bond, and an anion-exchange resin with M-TCPP by ion-exchange reaction and physical adsorption. The carriers modified with Co(3+)-TCPP proved to accelerate the redox reaction which is catalyzed by glutathione oxidase (GSHOx), while those modified with Mn(3+)-TCPP exhibited no activity. Formation of GS-SG and hydrogen peroxide was confirmed by means of mass spectroscopy and colored reaction, respectively. The silica gel modified with Co(3+)-TCPP exhibited the strongest activity among the tested carriers, and was expected to be useful practically as a solid catalyst for the determination of glutathione.
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Banco de datos:
MEDLINE
Idioma:
En
Revista:
Talanta
Año:
1998
Tipo del documento:
Article
País de afiliación:
Japón