Detection of Anaplasma antibodies in wildlife and domestic species in wildlife-livestock interface areas of Kenya by major surface protein 5 competitive inhibition enzyme-linked immunosorbent assay.
Onderstepoort J Vet Res
; 75(3): 199-205, 2008 Sep.
Article
en En
| MEDLINE
| ID: mdl-19040134
ABSTRACT
The seroprevalence of Anaplasma antibodies in wildlife (eland, blue wildebeest, kongoni, impala, Thomson's gazelle, Grant's gazelle, giraffe and plains zebra) and domestic animal (cattle, sheep and goat) populations was studied in wildlife/livestock interface areas of Kenya. Serum samples were analyzed by competitive inhibition enzyme-linked immunosorbent assay (CI-ELISA), using a recombinant antigen (MSP-5) from Anaplasma marginale surface membrane. A monoclonal antibody, FC-16, was used as the primary antibody, while anti-mouse conjugated to horseradish peroxidase was used as the secondary antibody. The results indicate a high seroprevalence in both wildlife and livestock populations, in contrast to earlier reports from Kenya, which indicated a low seroprevalence. The differences are attributed to the accurate analytical method used (CI-ELISA), as compared with agglutination techniques, clinical signs and microscopy employed by the earlier workers.
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Banco de datos:
MEDLINE
Asunto principal:
Proteínas de la Membrana Bacteriana Externa
/
Ensayo de Inmunoadsorción Enzimática
/
Anaplasma
/
Anaplasmosis
/
Anticuerpos Antibacterianos
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
País/Región como asunto:
Africa
Idioma:
En
Revista:
Onderstepoort J Vet Res
Año:
2008
Tipo del documento:
Article
País de afiliación:
Kenia