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Elevated ATG5 expression in autoimmune demyelination and multiple sclerosis.
Alirezaei, Mehrdad; Fox, Howard S; Flynn, Claudia T; Moore, Craig S; Hebb, Andrea L O; Frausto, Ricardo F; Bhan, Virender; Kiosses, William B; Whitton, J Lindsay; Robertson, George S; Crocker, Stephen J.
Afiliación
  • Alirezaei M; Molecular and Integrative Neurosciences Department, The Scripps Research Institute, La Jolla, California 92037, USA. malireza@scripps.edu
Autophagy ; 5(2): 152-8, 2009 Feb.
Article en En | MEDLINE | ID: mdl-19066443
Multiple sclerosis (MS) is an inflammatory central nervous system (CNS) disorder characterized by T cell-mediated demyelination. In MS, prolonged T cell survival and increased T cell proliferation have been linked to disease relapse and progression. Recently, the autophagy-related gene 5 (Atg5) has been shown to modulate T cell survival. In this study, we examined the expression of Atg5 using both a mouse model of autoimmune demyelination as well as blood and brain tissues from MS cases. Quantitative real-time PCR analysis of RNA isolated from blood samples of experimental autoimmune encephalomyelitis (EAE) mice revealed a strong correlation between Atg5 expression and clinical disability.Analysis of protein extracted from these cells confirmed both upregulation and post-translational modification of Atg5, the latter of which was positively correlated with EAE severity. Analysis of RNA extracted from T cells isolated by negative selection indicated that Atg5 expression was significantly elevated in individuals with active relapsing-remitting MS compared to non-diseased controls. Brain tissue sections from relapsing-remitting MS cases examined by immunofluorescent histochemistry suggested that encephalitogenic T cells are a source of Atg5 expression in MS brain samples. Together these data suggest that increased T cell expression of Atg5 may contribute to inflammatory demyelination in MS.
Asunto(s)

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Encefalomielitis Autoinmune Experimental / Proteínas Asociadas a Microtúbulos / Esclerosis Múltiple Límite: Adult / Aged / Animals / Female / Humans / Male / Middle aged Idioma: En Revista: Autophagy Año: 2009 Tipo del documento: Article País de afiliación: Estados Unidos

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Encefalomielitis Autoinmune Experimental / Proteínas Asociadas a Microtúbulos / Esclerosis Múltiple Límite: Adult / Aged / Animals / Female / Humans / Male / Middle aged Idioma: En Revista: Autophagy Año: 2009 Tipo del documento: Article País de afiliación: Estados Unidos